FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513485
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA28290.5509411180462915No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC24470.47654751355930547No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC22170.43175555274253385No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17550.34178213579754035No Hit
ATATAGGAGAGGCAGTGGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG16110.31373847337312677No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15640.3045853335540473No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC14100.2745941945723828No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13480.262519839917427No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12140.23642365405026436No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA11800.22980223375561118No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11700.2278547571983602No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT11430.22259657049378268No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTACAAG11340.2208438415922568No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10700.20837999162585083No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGTCAGTC10460.20370604788844854No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC10400.202537561954098No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG10060.1959161416594448No Hit
CCCTTATCTAATGGCAAATATTTAGTAACCTTTGGATAAAACCTAGCAGG10060.1959161416594448No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC10030.1953318986922695No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT9960.19396866510219382No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA9740.18968421667624177No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT9220.17955733857853687No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT9060.17644137608693536No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT9020.17566238546403498No Hit
ATCCAGCATCTAGGGATCTAGTAGTCAGTTATGTCAACACTCATATGGGC9000.1752728901525848No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTAGTAACCTTTGGATAAAAC8690.16923571282510685No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA8510.16573025502205516No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA8390.16339328315335405No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT8370.16300378784190384No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8260.1608615636289278No Hit
TCTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG7950.1548243863014499No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA7630.14859246131824688No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC7580.14761872303962142No Hit
CTCCTATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG7480.14567124648237048No Hit
TACCTTGATAGTCCAGTAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7380.1437237699251195No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT7340.14294477930221913No Hit
AGATTGAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCG7310.14236053633504386No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7210.14041305977779292No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7160.13943932149916743No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7080.1378813402533667No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC7070.1376865925976416No Hit
TCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA7040.13710234963046633No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG6960.13554436838466558No Hit
ATGCTGGATCGTCCAAATTAGCACCCACCCAGGTAGCTAGAGTCATTAGT6930.13496012541749028No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6930.13496012541749028No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6650.12950719105718764No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT6620.12892294809001237No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6560.1277544621556618No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACACACGGGCAAC6550.12755971449993672No Hit
ATCAAGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATCTTCAACCACC6540.1273649668442116No Hit
CTATGTATCCCTCCTGTTGTTGTACCAAACCTTCGGACGGAAATTGCACC6530.12717021918848653No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC6460.12580698559841086No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6400.12463849966406028No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6320.12308051841825954No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTGAAACATTCTTTGATTTTCTGT6240.12152253717245878No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT6160.11996455592665804No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT6070.11821182702513218No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5980.11645909812360634No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5950.11587485515643105No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT5940.11568010750070597No Hit
CAATTCTCTAGGGGGAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCC5800.11295364032055465No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC5800.11295364032055465No Hit
GTCCAGTAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5790.11275889266482955No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5750.11197990204192917No Hit
GTACAGTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAA5740.11178515438620407No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5660.11022717314040333No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5610.10925343486177785No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG5550.10808494892742729No Hit
AGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGCGGTATTGTGAG5530.10769545361597711No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTAGTAACCTTTGGATAAAA5500.10711121064880182No Hit
GTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAAGGAAT5480.10672171533735163No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5470.10652696768162653No Hit
TCTCCATCTCGGGGATCTCAATGTTAGTATTCCTTGGACTCATAAAGTGG5420.10555322940300108No Hit
CTCCTGTTGTTGTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCA5410.10535848174727597No Hit
GAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA5410.10535848174727597No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG5400.10516373409155087No Hit
GACCTGGCCGTTGCCTAGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5360.1043847434686505No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT5360.1043847434686505No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5300.10321625753429993No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGATCCCCGAGATTGAGATCT5280.10282676222284975No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5270.10263201456712463No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC5240.10204777159994938No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5220.10165827628849917No Hit
CCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGC5170.10068453800987372No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGG5140.10010029504269842No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT5140.10010029504269842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)