FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504702
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC27500.544875986225535No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTACGT22460.44501507820456426No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21190.4198517144770577No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20810.4123225190310322No Hit
CCTCCCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19850.3933013936937044No Hit
CAAGATATACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG19550.38735729202578945No Hit
GTATATTGCCCGTTTGTCCTCTAATTCCAGGATCATCCACAACCAGCACC16780.3324734199587083No Hit
GGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG14700.2912609817278315No Hit
GATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTAAAAGCC14030.2779858213361548No Hit
GTATATCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTACGT12320.2441044418290397No Hit
AACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGC11230.22250753910228213No Hit
GTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC10810.21418579676720123No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10780.21359138660040974No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10750.21299697643361826No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT10120.20051436293099692No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10060.19932554259741395No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9630.1908056635400692No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT9430.18684292909479258No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT9250.1832764680940436No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8810.17455845231443504No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8590.17019944442463078No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG8330.16504788964577116No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8300.16445347947897967No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGA8170.16187770208954988No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC8110.1606888817559669No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7900.15652801058842644No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7730.1531596863099413No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7670.1519708659763583No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7640.15137645580956682No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7600.1505839089205115No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAA7580.15018763547598385No Hit
GAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTCACGGTG7580.15018763547598385No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7560.14979136203145618No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7550.14959322530919233No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7470.1480081315310817No Hit
ATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACC7460.14780999480881787No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7360.14582862758617957No Hit
CTTGACTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGCATA7190.14246030330769444No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7160.14186589314090295No Hit
GTACAAAACCTACGGACAGAAACTGCACCTGTATTCCCATCCCATCATCT7150.14166775641863913No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6820.13512924458393272No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6720.1331478773612944No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6690.1325534671945029No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6630.13136464686091992No Hit
CAAGAGATACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG6500.1287888694714901No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6410.12700563897111564No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6340.12561868191526881No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACAGAAT6340.12561868191526881No Hit
GACAGAAACTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAA6330.12542054519300497No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6320.12522240847074115No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG6310.1250242717484773No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6280.12442986158168583No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6180.12244849435904752No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCCGTGGA6050.11987271696961771No Hit
GTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCCACAACCAGCACC5960.11808948646924322No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG5940.11769321302471558No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5930.11749507630245173No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5930.11749507630245173No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5920.11729693958018791No Hit
GTCCGGTGCTGGTTGTGGATGATCCTGGAATTAGAGGACAAACGGGCAAC5910.11709880285792407No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGCGGAGGTTA5910.11709880285792407No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5860.11610811924660491No Hit
GGTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGT5850.11590998252434109No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTTTCGCCAACTTACAAGGCC5820.1153155723575496No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5780.11452302546849427No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5770.11432488874623045No Hit
GTACAGGGTCCCCAGTCTTCGATGAGATTGACGATATGGCAGAGACAGTA5750.11392861530170278No Hit
GAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGACCCTG5730.11353234185717512No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCATATAGACCACCAAATGCC5710.11313606841264745No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5680.11254165824585596No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAAA5680.11254165824585596No Hit
GTATACATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5650.11194724807906448No Hit
GATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTAAAAGCC5630.11155097463453681No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5560.11016401757869002No Hit
GTTTTGTACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC5540.10976774413416235No Hit
GGCAATATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG5470.10838078707831553No Hit
AAGATATACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGG5410.10719196674473254No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5390.10679569330020487No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT5370.10639941985567722No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5360.10620128313341338No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA5320.10540873624435805No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5300.1050124627998304No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5270.1044180526330389No Hit
AGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTC5240.10382364246624741No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA5230.10362550574398358No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5140.1018422752436091No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT5110.10124786507681761No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5050.10005904474323461No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTT5050.10005904474323461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)