FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504702
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC26010.5153536146082243No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20760.411331835419713No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTACGT20370.4036045032514236No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18560.3677417565216702No Hit
CAAGATATACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG18140.3594200141865893No Hit
GTATATTGCCCGTTTGTCCTCTAATTCCAGGATCATCCACAACCAGCACC16970.33623801768172107No Hit
CCTCCCGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14940.2960162630621634No Hit
GATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTAAAAGCC13130.26015351633241No Hit
GTATATCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTACGT11980.23736779327206947No Hit
GGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG11950.23677338310527796No Hit
AACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGC11200.22191312893549064No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10680.21161001937777144No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10400.20606219115438415No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT10270.20348641376495435No Hit
GTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC9590.19001311665101386No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9210.1824839212049883No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8940.17713422970386486No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT8930.176936092981601No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC8650.17138826475821375No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8560.1696050342578393No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8220.16286838570086903No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGA8100.16049074503370306No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG7910.15672614731069026No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7570.14998949875372No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT7440.1474137213642902No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7400.1466211744752349No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7320.14503608069712423No Hit
GTACAAAACCTACGGACAGAAACTGCACCTGTATTCCCATCCCATCATCT7310.1448379439748604No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7130.14127148297411146No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7130.14127148297411146No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7120.14107334625184761No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7100.14067707280731997No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6970.13810129541789015No Hit
CAAGAGATACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG6960.13790315869562633No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6950.1377050219733625No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6780.13433669769487738No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6740.13354415080582205No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6680.13235533047223907No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGCGGAGGTTA6550.12977955308280928No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6530.1293832796382816No Hit
GACAGAAACTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAA6500.1287888694714901No Hit
GAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTCACGGTG6430.1274019124156433No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCCGTGGA6370.1262130920820603No Hit
CTTGACTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGCATA6360.12601495535979645No Hit
GTATACATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6350.12581681863753263No Hit
ATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACC6320.12522240847074115No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6310.1250242717484773No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6280.12442986158168583No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6260.12403358813715816No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAA6240.12363731469263049No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6200.12284476780357519No Hit
GAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGACCCTG6050.11987271696961771No Hit
GTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCCACAACCAGCACC6010.11908017008056239No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5970.11828762319150707No Hit
GATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTAAAAGCC5870.11630625596886876No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACAGAAT5800.11491929891302194No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5740.11373047857943896No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTTTCGCCAACTTACAAGGCC5670.11234352152359214No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5640.11174911135680066No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5590.1107584277454815No Hit
GGTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGT5590.1107584277454815No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5570.11036215430095384No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5510.10917333396737086No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5450.10798451363378786No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT5420.10739010346699637No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5370.10639941985567722No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAAA5360.10620128313341338No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG5330.10560687296662188No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5230.10362550574398358No Hit
GTTTTGTACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC5190.10283295885492826No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG5130.10164413852134525No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5070.10045531818776228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)