FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272278
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15200.558252962046144No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14750.5417257361960937No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14580.5354821175416302No Hit
CCTCCTGCATATAGACCACCCAATGCCCCTATCTTATCAACACTTCCGGA10410.38232982466449733No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6500.2387265956118379No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6370.23395206369960114No Hit
ATATGTTCCTGTGGCAATGTTCCCCAACTCCCAATTACATATCCCATGAA6270.23027934684403442No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5940.21815938122066417No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5930.2177921095351075No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5640.20714123065396395No Hit
CTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTG5560.20420305716951057No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5550.20383578548395392No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5230.19208309154614034No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5130.18841037469057362No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5070.18620674457723357No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5050.1854722012061202No Hit
GCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT5000.18363584277833683No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4860.1784940391805434No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT4670.17151587715496663No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT4650.17078133378385327No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4590.16857770367051322No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4520.1660068018716165No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4490.1649049868149465No Hit
GTATACATGCATATAAAGGCATTAAGGCCGGATATCCACATTGCGTAAAA4360.16013045490270972No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA4250.1560904663615863No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4230.15535592299047296No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4200.15425410793380295No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4170.15315229287713295No Hit
ATCTCAATGTTAGTATTCCGTGGACACATAAGGTGGGAAACTTTACGGGG4110.15094866276379287No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4100.15058139107823623No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4090.15021411939267953No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4060.14911230433600953No Hit
GTACAAGAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4060.14911230433600953No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4020.14764321759378282No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG4010.14727594590822615No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT4010.14727594590822615No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3870.14213414231043273No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT3820.14029778388264935No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3820.14029778388264935No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA3800.139563240511536No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3670.13478870859929926No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT3600.13221780680040254No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA3600.13221780680040254No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3600.13221780680040254No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3520.12927963331594913No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT3510.12891236163039246No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3490.12817781825927912No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA3430.12597418814593908No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3420.1256069164603824No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG3390.12450510140371238No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3360.12340328634704235No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG3310.121566927919259No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3290.12083238454814564No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3270.1200978411770323No Hit
GACTAAGGCCCACTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGA3260.11973056949147562No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3240.11899602612036228No Hit
CAATAGGCCTATTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT3210.11789421106369224No Hit
TATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG3210.11789421106369224No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTC3170.11642512432146557No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3100.11385422252256884No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT3070.11275240746589882No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG3060.11238513578034216No Hit
TCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAG3030.11128332072367211No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG2990.10981423398144545No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG2980.10944696229588877No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT2970.10907969061033208No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG2960.1087124189247754No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2950.10834514723921874No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC2940.10797787555366206No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2920.10724333218254872No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2920.10724333218254872No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG2900.10650878881143537No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC2890.10614151712587869No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA2880.10577424544032203No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT2870.10540697375476535No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC2860.10503970206920868No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA2860.10503970206920868No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT2840.10430515869809534No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT2820.10357061532698197No Hit
GTGGATATCCGGCCTTAATGCCTTTATATGCATGTATACAAGCAAAACAG2810.10320334364142532No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC2810.10320334364142532No Hit
ATGTGGATATCCGGCCTTAATGCCTTTATATGCATGTATACAAGCAAAAC2810.10320334364142532No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC2800.10283607195586864No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGACGGTCGGTACAA2790.10246880027031195No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA2790.10246880027031195No Hit
GCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCC2790.10246880027031195No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA2790.10246880027031195No Hit
CTCTTGTACCGACCGTCCACGGGGCGCACCTCTCTTTACGCGGACTCCCC2790.10246880027031195No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2770.10173425689919861No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC2770.10173425689919861No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA2730.10026517015697192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)