FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272278
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15100.5545802451905774No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14580.5354821175416302No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12310.4521114449202653No Hit
CCTCCTGCATATAGACCACCCAATGCCCCTATCTTATCAACACTTCCGGA8470.31107911766650265No Hit
ATATGTTCCTGTGGCAATGTTCCCCAACTCCCAATTACATATCCCATGAA6370.23395206369960114No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6190.22734117335958104No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5530.20310124211284056No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5390.19795943851504713No Hit
CTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTG5300.19465399334503705No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5290.19428672165948038No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5250.1928176349172537No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5090.1869412879483469No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4920.18069766929388345No Hit
GTACAAGAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4840.17775949580943007No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT4680.17188314884052328No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4660.17114860546940994No Hit
GTATACATGCATATAAAGGCATTAAGGCCGGATATCCACATTGCGTAAAA4620.16967951872718323No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT4600.1689449753560699No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4540.16674134524272985No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4480.1645377151293898No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT4400.16159954164493642No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA4330.1590286398460397No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA4320.158661368160483No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4220.1549886513049163No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4220.1549886513049163No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT4200.15425410793380295No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT4110.15094866276379287No Hit
ATCTCAATGTTAGTATTCCGTGGACACATAAGGTGGGAAACTTTACGGGG4080.14984684770712287No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4070.1494795760215662No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4030.14801048927933952No Hit
GCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT4020.14764321759378282No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG3990.1465414025371128No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG3920.1439705007382161No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG3840.1410323272537627No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3810.13993051219709268No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA3800.139563240511536No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3710.13625779534152593No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3700.13589052365596926No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG3660.1344214369137426No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA3560.13074872005817584No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC3560.13074872005817584No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3510.12891236163039246No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3490.12817781825927912No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT3450.12670873151705242No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3440.12634145983149575No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA3370.12377055803259904No Hit
CTCTTGTACCGACCGTCCACGGGGCGCACCTCTCTTTACGCGGACTCCCC3330.12230147129037233No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT3310.121566927919259No Hit
GTGGATATCCGGCCTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3290.12083238454814564No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3250.11936329780591895No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA3240.11899602612036228No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG3230.11862875443480561No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC3210.11789421106369224No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3180.11679239600702222No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3170.11642512432146557No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3140.11532330926479553No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC3130.11495603757923888No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3120.11458876589368219No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3120.11458876589368219No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG3080.1131196791514555No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3070.11275240746589882No Hit
ATGTGGATATCCGGCCTTAATGCCTTTATATGCATGTATACAAGCAAAAC3070.11275240746589882No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3020.11091604903811546No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT3020.11091604903811546No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3010.11054877735255877No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC3010.11054877735255877No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT2990.10981423398144545No Hit
TCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAG2990.10981423398144545No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT2990.10981423398144545No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG2980.10944696229588877No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG2930.10761060386810539No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT2920.10724333218254872No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2920.10724333218254872No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2910.10687606049699205No Hit
CAATAGGCCTATTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT2900.10650878881143537No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA2900.10650878881143537No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA2880.10577424544032203No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC2870.10540697375476535No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT2840.10430515869809534No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2830.10393788701253866No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG2810.10320334364142532No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG2750.10099971352808526No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCCAATGCC2750.10099971352808526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)