FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451940
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17500.387219542417135No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA14330.3170774881621454No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT12540.2774704606806213No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT12450.2754790458910475No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11480.2540160198256406No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10250.22680001770146477No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT10070.22281718812231713No Hit
TCCCCGAAGAATCCCAGGGGATTGGGGACAGAAAGATTTGTCCCCATGCC10010.22148957826260124No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9320.20622206487586847No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9290.20555825994601054No Hit
ATCTCAATGTTAGTATTCCTTGGACGCATAAGGTGGGAAACTTTACGGGG9100.2013541620569102No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9090.20113289374695756No Hit
CTTCCCCGATCATCAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATC8800.19471611275833076No Hit
GTATCGGGAAGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8470.18741425852989335No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8300.18365269726069833No Hit
TCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGATTTGTCCCCATGCC7660.1694915254237288No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAGTATTTAGTAACATT7590.16794264725406027No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA7490.16572996415453378No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7360.16285347612514936No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7110.15732176837633313No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAGTA7040.1557728902066646No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTTA7020.15533035358675928No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7020.15533035358675928No Hit
ACTAAATACTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTA7010.15510908527680667No Hit
GTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC6940.15356020710713814No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6900.15267513386732753No Hit
GACCTTTAACCTAACCTCCTCCCCTAACTCCTCCCACTCATTAAACACAC6790.15024118245784837No Hit
ATCTTAATGTTAGTATTCCTTGGACGCATAAGGTGGGAAACTTTACGGGG6740.14913484090808515No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6650.1471434261185113No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAAAGCACGACAGAAT6550.14493074301898484No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6540.14470947470903217No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTTAAAGTCCAA6530.14448820639907953No Hit
TTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAATCCGCACAAGGA6450.14271805991945835No Hit
GATGTATACTTGCGGGAAAGCACGACAGAATTGTCAGTCCCGATGAGTTT6440.14249679160950568No Hit
GCACCGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG6330.14006284020002654No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6300.13939903527016861No Hit
GAATTAATAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA6270.13873523034031066No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC6260.138513962030358No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATGATTAACTA6230.13785015710050008No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA6220.1376288887905474No Hit
ATATTAACGTTGACATAGCTGACTATTAATTCCCTGGATGCTGGATCTTC6190.13696508386068948No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6160.13630127893083155No Hit
TGCTTAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGATTCCCGT6120.13541620569102092No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG6090.134752400761163No Hit
CTCCTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6060.13408859583130506No Hit
ATCCCCGAAGAATCCCAGGGGATTGGGGACAGAAAGATTTGTCCCCATGC6060.13408859583130506No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6030.1334247909014471No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5970.1320971810417312No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5930.1312121078019206No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5910.13076957118201532No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5870.12988449794220472No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5870.12988449794220472No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTTAGGCA5760.12745054653272558No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5740.1270080099128203No Hit
CTTTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGT5660.1252378634331991No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5610.12413152188343586No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACACGCAAAAC5600.1239102535734832No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5590.12368898526353056No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5510.12191883878390937No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCGGTG5500.12169757047395671No Hit
TTCATGGCCAACTCAGAAAATCCAGATTGGGACCTCAATCCGCACAAGGA5480.12125503385405141No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5430.12014869230428818No Hit
CTCCAAAAGACACTAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5410.11970615568438288No Hit
ATCTAAGGGCAAGTATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5390.11926361906447758No Hit
CATGTATACACGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5370.11882108244457229No Hit
CTTCGGGGATCATCAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATC5330.11793600920476169No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACACGCAAAACAG5280.11682966765499846No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCACCCAAAACCACATC5270.1166083993450458No Hit
GTATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTC5250.1161658627251405No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5170.11439571624551931No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5160.11417444793556666No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5160.11417444793556666No Hit
CTATTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTG5160.11417444793556666No Hit
CCACCAAATGCCCCTATCTTATCAACGCTTCCGGAAACTACTGTTGTTAG5110.11306810638580342No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5080.11240430145594547No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5000.1106341549763243No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4990.11041288666637165No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA4980.110191618356419No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA4970.10997035004646635No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4950.10952781342656104No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4940.1093065451166084No Hit
AGTTCATCATTATTTCCAGACGCGACATTATTTACACACTCTTTGGAAGG4910.10864274018675046No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT4870.10775766694693986No Hit
GGATATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4810.10643005708722397No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4790.10598752046731867No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGATTCCCGTGGGCGTTC4770.10554498384741336No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4770.10554498384741336No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACGGGAATCTGCCC4710.10421737398769748No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA4680.10355356905783955No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG4640.10266849581802893No Hit
ACTCACAACTGTGCCAGCAGTTCCTCCTCCTGCCTCCACCAATCGGCAGT4630.10244722750807629No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA4620.10222595919812363No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4610.102004690888171No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4600.10178342257821836No Hit
CAGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4590.1015621542682657No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA4580.10134088595831305No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4540.10045581271850244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)