FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451940
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17420.3854493959375138No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT12600.27879807054033723No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA12250.2710536796919945No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11350.25113953179625614No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10970.2427313360180555No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT10650.23565075009957076No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9580.21197504093463734No Hit
TCCCCGAAGAATCCCAGGGGATTGGGGACAGAAAGATTTGTCCCCATGCC9450.20909855290525292No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9290.20555825994601054No Hit
ATCTCAATGTTAGTATTCCTTGGACGCATAAGGTGGGAAACTTTACGGGG9020.19958401557728903No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT8790.1944948444483781No Hit
GTATCGGGAAGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8620.1907332831791831No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8530.18874186838960924No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7910.17502323317254503No Hit
CTTCCCCGATCATCAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATC7890.17458069655263972No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7650.16927025711377616No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAGTATTTAGTAACATT7360.16285347612514936No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA7310.16174713457538611No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7090.15687923175642784No Hit
ATCTTAATGTTAGTATTCCTTGGACGCATAAGGTGGGAAACTTTACGGGG6890.15245386555737486No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6870.15201132893746958No Hit
ATATTAACGTTGACATAGCTGACTATTAATTCCCTGGATGCTGGATCTTC6810.15068371907775369No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTTAAAGTCCAA6760.14957737752799044No Hit
TCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGATTTGTCCCCATGCC6610.1462583528787007No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6600.14603708456874806No Hit
ACTAAATACTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTA6590.14581581625879542No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6530.14448820639907953No Hit
GTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC6460.142939328229411No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAAAGCACGACAGAAT6400.14161171836969508No Hit
GAATTAATAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA6380.1411691817497898No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6380.1411691817497898No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6360.1407266451298845No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG6310.13962030358012126No Hit
GACCTTTAACCTAACCTCCTCCCCTAACTCCTCCCACTCATTAAACACAC6250.13829269372040537No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6200.13718635217064212No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATGATTAACTA6200.13718635217064212No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAGTA6140.13585874231092623No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC6130.1356374740009736No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6090.134752400761163No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTTA6060.13408859583130506No Hit
GATGTATACTTGCGGGAAAGCACGACAGAATTGTCAGTCCCGATGAGTTT6040.13364605921139974No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5990.1325397176616365No Hit
CTCCTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5910.13076957118201532No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA5880.13010576625215736No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5840.1292206930123468No Hit
ATCCCCGAAGAATCCCAGGGGATTGGGGACAGAAAGATTTGTCCCCATGC5780.12789308315263087No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5690.12590166836305705No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5670.12545913174315174No Hit
GTATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTC5660.1252378634331991No Hit
TTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAATCCGCACAAGGA5660.1252378634331991No Hit
GCACCGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG5600.1239102535734832No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5580.1234677169535779No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5420.11992742399433554No Hit
CTTCGGGGATCATCAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATC5400.11948488737443022No Hit
TGCTTAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGATTCCCGT5380.11904235075452495No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA5360.11859981413461965No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTTAGGCA5360.11859981413461965No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACACGCAAAACAG5360.11859981413461965No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5340.11815727751471435No Hit
CTCCAAAAGACACTAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5320.11771474089480903No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5300.11727220427490376No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5260.11638713103509316No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCGGTG5250.1161658627251405No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5210.11528078948532991No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5210.11528078948532991No Hit
CTTTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGT5200.11505952117537727No Hit
CTATTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTG5190.11483825286542461No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCACCCAAAACCACATC5180.11461698455547197No Hit
ATCTAAGGGCAAGTATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5140.11373191131566138No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5130.11351064300570872No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5120.11328937469575608No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5050.11174049652608753No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5010.11085542328627693No Hit
TTCATGGCCAACTCAGAAAATCCAGATTGGGACCTCAATCCGCACAAGGA4990.11041288666637165No Hit
AGTTCATCATTATTTCCAGACGCGACATTATTTACACACTCTTTGGAAGG4910.10864274018675046No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4890.10820020356684516No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACACGCAAAAC4870.10775766694693986No Hit
CATGTATACACGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4870.10775766694693986No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA4800.10620878877727132No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA4790.10598752046731867No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4750.10510244722750808No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4720.10443864229765012No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTG4690.1037748373677922No Hit
CCACCAAATGCCCCTATCTTATCAACGCTTCCGGAAACTACTGTTGTTAG4680.10355356905783955No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4650.10288976412798159No Hit
GTATTATCCAGAGTATGTAGTTCATCATTATTTCCAGACGCGACATTATT4600.10178342257821836No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4590.1015621542682657No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT4580.10134088595831305No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4570.1011196176483604No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4570.1011196176483604No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4560.10089834933840774No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4560.10089834933840774No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGATTCCCGTGGGCGTTC4530.10023454440854981No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4520.10001327609859717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)