FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319060
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA21970.6885852190810506No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18570.5820221901836645No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17590.5513069642073591No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA10900.3416285338180906No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCG8270.25919889675923025No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8160.2557512693537266No Hit
ATATGTTCCTGCGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7690.2410204977120291No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7630.23913997367266343No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT7620.23882655299943584No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7450.23349840155456655No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7430.2328715602081113No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6680.20936500971604088No Hit
ACTCACAACTGTGCCAGCAACTCCTCCTCCTGCCTCCACCAATCGGCAGT6540.20497712029085438No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6440.2018429135585783No Hit
GCACCGGACCATGCAAAACCTGCACAGCTCCTGCTCAAGGAACCTCTATG6370.1996489688459851No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6360.19933554817275745No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6290.19714160346016424No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5960.18679872124365324No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5940.18617187989719802No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5940.18617187989719802No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5870.18397793518460476No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT5870.18397793518460476No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5790.18147056979878393No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5770.1808437284523287No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5690.17833636306650785No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5660.17739610104682504No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5590.1752021563342318No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5560.174261894314549No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5430.1701874255625901No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5420.1698740048893625No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5380.1686203221964521No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5330.16705321883031404No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5260.1648592741177208No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGT5260.1648592741177208No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5250.16454585344449318No Hit
GGATATCCACATTGCGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAAT5240.1642324327712656No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5160.16172506738544476No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5090.1595311226728515No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT5050.15827743997994106No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4910.1538895505547546No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA4890.15326270920829937No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4890.15326270920829937No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4810.15075534382247852No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT4790.1501285024760233No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT4760.1491882404563405No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4750.1488748197831129No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4720.1479345577634301No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATGAGAGGACAAACGGGCAAC4660.14605403372406445No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCGCAG4640.14542719237760923No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4630.1451137717043816No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4610.1444869303579264No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4580.1435466683382436No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG4580.1435466683382436No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT4530.14197956497210557No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4460.13978562025951233No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4450.1394721995862847No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4440.1391587789130571No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA4440.1391587789130571No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA4420.1385319375666019No Hit
AGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCG4410.13821851689337428No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4370.13696483420046385No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4330.13571115150755345No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4320.13539773083432585No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA4300.13477088948787064No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCGCAGGAACATATTGTACAAAAA4210.13195010342882216No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGCCT4180.13100984140913935No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGGAAGGCCTTGTAAGTTGGCGA4170.13069642073591173No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCTGCA4170.13069642073591173No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4170.13069642073591173No Hit
GGTTGGGGCTTGGCCATCGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4150.1300695793894565No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAGCTCTTCAAG4120.1291293173697737No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4090.1281890553500909No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG3980.12474142794458723No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA3950.12380116592490441No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCTTCCTGC3940.12348774525167681No Hit
GTTCAGATACTGTTTACTTAGGAAGGCCTTGTAAGTTGGCGAGAAAGTAA3930.12317432457844919No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3900.12223406255876638No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA3880.12160722121231117No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3840.12035353851940075No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTC3820.11972669717294554No Hit
GTAATTGGGAGTTGGGGCACATTGCCGCAGGAACATATTGTACAAAAAAT3810.11941327649971792No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG3770.11815959380680749No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA3760.1178461731335799No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTCCTAAGTAAACAGTATC3750.11753275246035229No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT3730.11690591111389707No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC3700.11596564909421427No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3690.11565222842098664No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA3670.11502538707453143No Hit
GGTTCAGATACTGTTTACTTAGGAAGGCCTTGTAAGTTGGCGAGAAAGTA3670.11502538707453143No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG3670.11502538707453143No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG3650.11439854572807623No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT3650.11439854572807623No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC3630.11377170438162101No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3620.11345828370839342No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3560.11157775966902778No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3560.11157775966902778No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3510.11001065630288974No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3490.10938381495643452No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3460.10844355293675172No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC3450.1081301322635241No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA3450.1081301322635241No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3430.1075032909170689No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3430.1075032909170689No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG3430.1075032909170689No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3420.1071898702438413No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA3420.1071898702438413No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGATGGTCGGTACAA3400.10656302889738609No Hit
TATCCACATTGCGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCG3400.10656302889738609No Hit
ACATAGAGGTTCCTTGAGCAGGAGCTGTGCAGGTTTTGCATGGTCCGGTG3400.10656302889738609No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTG3380.10593618755093087No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT3350.10499592553124804No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC3300.10342882216511003No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT3300.10342882216511003No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT3300.10342882216511003No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3280.10280198081865481No Hit
CTGTTTACTTAGGAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT3270.10248856014542719No Hit
CAATAGGCCGGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT3260.1021751394721996No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT3250.10186171879897198No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTC3250.10186171879897198No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3230.10123487745251677No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG3220.10092145677928915No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT3220.10092145677928915No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG3200.10029461543283394No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT3200.10029461543283394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)