FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446805
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24570.5499043206768054No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT18010.4030841194704625No Hit
GTGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCTAAGC17960.4019650630588288No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT17540.3925649892011056No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15230.3408645829836282No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14840.3321359429728853No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12170.2723783305916451No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11580.2591734649343673No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA10260.2296303756672374No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10030.2244827161737223No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9940.22246841463278164No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9640.2157540761629794No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9570.2141873971866922No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT9280.20769686999921663No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9210.20613019102292945No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8840.19784917357684No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT8600.1924777028009982No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8590.19225389151867145No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8570.19180626895401795No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACC8560.19158245767169124No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8440.18889672228377033No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8030.1797204597083739No Hit
CCCACATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA7630.17076800841530423No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT7620.17054419713297747No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG7580.1696489520036705No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7450.16673940533342285No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7300.16338223609852173No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT7280.16293461353386823No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG7250.16226317968688803No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGAACTTTACCACAG7140.15980125558129385No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACACTGTGTAAAA7010.1568917089110462No Hit
GGATTAAAGATAGGCACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6910.1546535960877788No Hit
CGATATGGGTGAGGCAATAGTCGGAACAGGGTTTACTGTTCCGGAACTGG6690.14972974787659046No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA6680.14950593659426373No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTG6610.14793925761797652No Hit
GATAAGATAGGGGCATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCA6440.1441344658184219No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT6430.14391065453609517No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6420.14368684325376843No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6360.14234397555980796No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6230.1394344288895603No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6190.13853918376025334No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC6040.13518201452535222No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA6000.13428676939604525No Hit
ATTCTTTGACATACCTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG6000.13428676939604525No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6000.13428676939604525No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5970.13361533554906502No Hit
TATCCTATCAACACTTCCGGAAACTACTGTTGTTAGATGCCGAGGCAGGT5970.13361533554906502No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA5940.1329439017020848No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5930.13272009041975807No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5760.12891529862020346No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5590.12511050682064884No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5530.12376763912668838No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAACCTCTATG5530.12376763912668838No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA5480.12264858271505466No Hit
CCACCAAATGCCCCTATCCTATCAACACTTCCGGAAACTACTGTTGTTAG5450.12197714886807444No Hit
TTCTTCTACTGTGCCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5420.12130571502109422No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5410.12108190373876747No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5320.11906760219782678No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATG5230.11705330065688611No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC5230.11705330065688611No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5100.11414375398663845No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5100.11414375398663845No Hit
GGTGCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTG5090.11391994270431173No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG5060.11324850885733151No Hit
CTATCAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4980.11145801859871755No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4930.11033896218708385No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG4900.10966752834010363No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4870.1089960944931234No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG4840.10832466064614318No Hit
GCACTCAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4800.1074294155168362No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4780.10698179295218271No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4750.10631035910520249No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG4740.10608654782287574No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4730.10586273654054901No Hit
ATATTGTACTGAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA4720.10563892525822227No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA4670.10451986884658857No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4630.1036246237172816No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4600.10295318987030137No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4570.10228175602332112No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4570.10228175602332112No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGGAGTTCCACTGCATG4560.10205794474099439No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4520.10116269961168742No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4510.10093888832936068No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4470.10004364320005371No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4470.10004364320005371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)