FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446805
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24540.5492328868298252No Hit
GTGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCTAAGC17800.39838408254160096No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT17150.3838363491903627No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT16620.37197435122704536No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15860.35496469377021295No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12110.27103546289768465No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11930.26700685981580335No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10660.23858282696030708No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10160.22739226284397No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9940.22246841463278164No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9150.204787323328969No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9010.2016539653763946No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8950.20031109768243416No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACC8780.19650630588287954No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT8590.19225389151867145No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8250.1846443079195622No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8180.18307762894327503No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8010.17927283714372041No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT7950.17792996944975997No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7540.16875370687436353No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGAACTTTACCACAG7410.1658441602041159No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7090.15868219916966014No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7080.1584583878873334No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6940.155325029934759No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACACTGTGTAAAA6940.155325029934759No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT6870.15375835095847182No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA6680.14950593659426373No Hit
CGATATGGGTGAGGCAATAGTCGGAACAGGGTTTACTGTTCCGGAACTGG6570.14704401248866955No Hit
GGATTAAAGATAGGCACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6430.14391065453609517No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6420.14368684325376843No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6410.14346303197144167No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6390.1430154094067882No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG6380.14279159812446146No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6370.14256778684213472No Hit
CCCACATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA6340.14189635299515446No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCGTCTGCCGGTCCGTGTG6170.13809156119559987No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT6170.13809156119559987No Hit
GATAAGATAGGGGCATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCA6140.13742012734861964No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6110.13674869350163943No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6090.13630107093698593No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6000.13428676939604525No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5990.13406295811371852No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTG5990.13406295811371852No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5900.13204865657277784No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5730.12824386477322322No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5710.12779624220856975No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC5670.12690099707926278No Hit
ATTCTTTGACATACCTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG5660.12667718579693601No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA5640.12622956323228254No Hit
ATATTGTACTGAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA5620.12578194066762907No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA5620.12578194066762907No Hit
TATCCTATCAACACTTCCGGAAACTACTGTTGTTAGATGCCGAGGCAGGT5610.1255581293853023No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATG5420.12130571502109422No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5390.12063428117411398No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5380.12041046989178725No Hit
TTCTTCTACTGTGCCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5280.11817235706851983No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5260.11772473450386634No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5220.11682948937455936No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA5040.11280088629267801No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5040.11280088629267801No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAACCTCTATG5020.11235326372802452No Hit
CTATCAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5010.1121294524456978No Hit
GTACAATATGTTCCTGTGGTAAAGTTCCCCAACTTCCAATTACATATCCC5000.11190564116337104No Hit
CCACCAAATGCCCCTATCCTATCAACACTTCCGGAAACTACTGTTGTTAG4970.11123420731639082No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG4930.11033896218708385No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4900.10966752834010363No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4850.10854847192846992No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4840.10832466064614318No Hit
GCACTCAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4760.10653417038752923No Hit
GGTGCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTG4730.10586273654054901No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4640.10384843499960832No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4620.10340081243495486No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA4570.10228175602332112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)