FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332322
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15370.46250323481442696No Hit
GTACGGGACCATGCAAGACCTGCACGATTACTGCTCAAGGAACCTCTATG11200.3370225263449305No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC10580.318365922207979No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10390.31264857577891325No Hit
GTCCCGTACTGGTAGTTGACGTTCCTGGAAGTAGAGGACAAACGGGCAAC10390.31264857577891325No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9570.2879737122429451No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA9330.28075180096412516No Hit
GTATGTAAATAATGCCTAGTTTTGAAATAATGATTAACTGCATGTTCAGG8750.26329884870697695No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8740.2629979357370261No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8370.2518641558488454No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7970.23982763705081214No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT7970.23982763705081214No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC7870.23681850735130386No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7640.22989750904243478No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7500.22568472746312312No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7340.22087011994390982No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7300.21966646806410647No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7260.21846281618430316No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7170.21575459945474568No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6560.19739890828774503No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6510.19589434343799086No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6330.19047790997887593No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT6330.19047790997887593No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT6130.1844596505798593No Hit
CCTACCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5700.17152039287197357No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5680.1709185669320719No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5650.17001582802221943No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5480.1649003075330553No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC5290.1591829611039895No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5260.158280222194137No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5170.15557200546457953No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4920.14804918121580876No Hit
GTGGATGGCCGGAACGGCAGATGAAGAAGGGGACGGTAGAGGCCCAAACG4890.14714644230595628No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG4740.1426327477566938No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4670.140526356967038No Hit
GGGTTTACTGTTCCGAAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4670.140526356967038No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4630.13932270508723466No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4560.13721631429757886No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4540.1366144883576772No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4480.1348090105379722No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4470.13450809756802137No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4460.13420718459807054No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4390.13210079380841475No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG4380.13179988083846392No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4350.1308971419286114No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA4290.12909166410890643No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4260.12818892519905395No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4250.12788801222910312No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4230.12728618628920144No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACGTCAACTACC4210.12668436034929978No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4180.12578162143944727No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4180.12578162143944727No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4150.1248788825295948No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAACCTTAT4140.12457796955964397No Hit
CTATTAATAATATCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG4140.12457796955964397No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4130.12427705658969314No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGCCTCTGCCAAGTGTTTGCTG4120.12397614361974231No Hit
ACATAGAGGTTCCTTGAGCAGTAATCGTGCAGGTCTTGCATGGTCCCGTA4120.12397614361974231No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4060.12217066580003731No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4020.12096701392023398No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGCC4000.12036518798033233No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4000.12036518798033233No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3990.1200642750103815No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3980.11976336204043067No Hit
GTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTTGGGATAGAAC3960.11916153610052901No Hit
CTCCAAAAGATACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTAAGA3940.11855971016062733No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3920.11795788422072567No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3920.11795788422072567No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3910.11765697125077484No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3880.11675423234092236No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3850.11585149343106987No Hit
AATTCGCAGTCCCCAACCTCCAATCACTCACCAACCTCTTGTCCTCCAAT3850.11585149343106987No Hit
GGATGGGGCTTGGCCATTGGCCATCGGCGCATGCGTGGAACCTTTGTGGC3850.11585149343106987No Hit
CTCCTCCCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3830.11524966749116822No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3820.11494875452121739No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC3800.1143469285813157No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT3790.11404601561136488No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3780.11374510264141405No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3730.1122405377916599No Hit
ACTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT3680.11073597294190575No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3680.11073597294190575No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTGGGATAGAACCTAGCAGG3670.11043505997195491No Hit
TTATTATCCTGAACATGCAGTTAATCATTATTTCAAAACTAGGCATTATT3650.10983323403205324No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3620.10893049512220075No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTTCGGAACAGTAAACCCTGTTCCGA3610.10862958215224992No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT3590.10802775621234827No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3580.10772684324239744No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATAC3550.10682410433254495No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC3550.10682410433254495No Hit
GCATTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGA3500.10531953948279078No Hit
GGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTT3460.10411588760298747No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3450.10381497463303664No Hit
GTATTAAACCTTATTATCCTGAACATGCAGTTAATCATTATTTCAAAACT3440.10351406166308581No Hit
GGATTAAAGACAGGTACCGTAGAAGAATAGAGCCCAGTAAAGTTTCCCAC3440.10351406166308581No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3440.10351406166308581No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTACGG3430.10321314869313497No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3420.10291223572318414No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3420.10291223572318414No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG3410.10261132275323331No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3400.10231040978328249No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTTCGGA3340.10050493196357749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)