FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332322
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14100.4242872876306715No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10900.3279951372464056No Hit
GTACGGGACCATGCAAGACCTGCACGATTACTGCTCAAGGAACCTCTATG10580.318365922207979No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9590.28857553818284676No Hit
GTATGTAAATAATGCCTAGTTTTGAAATAATGATTAACTGCATGTTCAGG8700.2617942838572228No Hit
GTCCCGTACTGGTAGTTGACGTTCCTGGAAGTAGAGGACAAACGGGCAAC8660.2605906319774195No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8660.2605906319774195No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA8230.24765137426953376No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8120.24434133160007462No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7600.2286938571626314No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7330.220569206973959No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7160.21545368648479488No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7150.21515277351484402No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6990.21033816599563077No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC6740.20281534174685997No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6740.20281534174685997No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT6730.20251442877690914No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6540.19679708234784338No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6330.19047790997887593No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6100.1835569116700068No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5920.17814047821089185No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT5830.17543226148133437No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5320.16008570001384198No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5180.15587291843453036No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC5120.15406744061482538No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5080.15286378873502207No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4960.1492528330956121No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT4960.1492528330956121No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4950.14895192012566127No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG4880.14684552933600545No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4840.1456418774562021No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4820.14504005151630045No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4680.14082726993698882No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC4660.14022544399708717No Hit
CCTACCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4660.14022544399708717No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4650.13992453102713634No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGCCTCTGCCAAGTGTTTGCTG4600.13841996617738217No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG4600.13841996617738217No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC4500.13541083647787386No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4450.13390627162811972No Hit
GTGGATGGCCGGAACGGCAGATGAAGAAGGGGACGGTAGAGGCCCAAACG4440.1336053586581689No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4390.13210079380841475No Hit
ACATAGAGGTTCCTTGAGCAGTAATCGTGCAGGTCTTGCATGGTCCCGTA4320.12999440301875892No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4190.12608253440939812No Hit
CTCCAAAAGATACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTAAGA4170.12548070846949644No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4090.1230734047098898No Hit
CTATTAATAATATCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG4050.12186975283008648No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4040.12156883986013566No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGCC4020.12096701392023398No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4010.12066610095028316No Hit
GTATTAAACCTTATTATCCTGAACATGCAGTTAATCATTATTTCAAAACT3980.11976336204043067No Hit
GGGTTTACTGTTCCGAAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT3980.11976336204043067No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3960.11916153610052901No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAACCTTAT3950.11886062313057816No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACGTCAACTACC3920.11795788422072567No Hit
AATTCGCAGTCCCCAACCTCCAATCACTCACCAACCTCTTGTCCTCCAAT3910.11765697125077484No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTTCGGAACAGTAAACCCTGTTCCGA3900.11735605828082402No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG3870.11645331937097153No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3860.1161524064010207No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3800.1143469285813157No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3790.11404601561136488No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3790.11404601561136488No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG3780.11374510264141405No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3780.11374510264141405No Hit
GGATGGGGCTTGGCCATTGGCCATCGGCGCATGCGTGGAACCTTTGTGGC3710.11163871185175823No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3700.1113377988818074No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3680.11073597294190575No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTACTCATCTGCCGGACCGTGTG3640.10953232106210241No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3640.10953232106210241No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3630.10923140809215158No Hit
GTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTTGGGATAGAAC3610.10862958215224992No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3570.10742593027244661No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3570.10742593027244661No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3560.10712501730249578No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3560.10712501730249578No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT3560.10712501730249578No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3550.10682410433254495No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTGGGATAGAACCTAGCAGG3540.10652319136259412No Hit
ATAATATCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTGCCAATC3470.1044168005729383No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3430.10321314869313497No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3410.10261132275323331No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA3400.10231040978328249No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA3400.10231040978328249No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3380.10170858384338081No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3350.10080584493352832No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3350.10080584493352832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)