FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317066
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16100.5077807144253879No Hit
ATATCTGAACCTTTACCCCGTTGCTCGGCAACGACCAGGTCTCTGCCAAG14680.4629950861965647No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA14100.44470236480732717No Hit
GTTCATATATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA12010.37878548945645385No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11310.35670806709013264No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11040.3481924898916945No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC10980.34630013940315263No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10820.3412538714337078No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8900.2806986558003696No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT8310.26209054266304177No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8270.26082897567068053No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8000.2523133984722424No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7870.24821330574706846No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7860.24789791399897812No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT7560.23843616155626904No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6830.21541256394567695No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6810.2147817804494963No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6420.20248150227397452No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6420.20248150227397452No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6410.2021661105258842No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6290.19838140954880054No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6260.19743523430452967No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6090.1920735745869945No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6040.19049661584654298No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTTCACCAAGCTCTGCTAG5900.18608113137327875No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5740.1810348634038339No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG5690.17945790466338238No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGTCT5680.1791425129152921No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5570.17567320368629874No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5550.17504242019011815No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5450.1718885027092151No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG5390.1699961522206733No Hit
CCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG5360.1690499769764024No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT5350.16873458522831208No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5300.16715762648786056No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5270.16621145124358966No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG5200.16400370900695754No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5160.16274214201459633No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5080.1602190080298739No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTG4920.15517274006042905No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4640.1463417711139006No Hit
CTTCCAGGAACATCAACTACCAGCACGGGGCCATGCAAGACCTGCACGAT4600.14508020412153938No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4440.1400339361520945No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4340.13688001867119148No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4200.13246453419792725No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4140.13057218370938542No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4080.12867983322084361No Hit
TCCTAGAAGTAGAGGACAAACGGGCAACATACCCTGGTAGTCCAGAAGAA4020.12678748273230178No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4000.1261566992361212No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3910.12331817350330845No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCATATTTCC3900.12300278175521816No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC3890.12268739000712785No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3760.1185872972819539No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT3730.11764112203768301No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT3710.11701033854150239No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCG3700.1166949467934121No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3660.11543337980105088No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3650.11511798805296058No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3640.11480259630487029No Hit
ATATATGAACCTTTACCCCGTTGCTCGGCAACGACCAGGTCTCTGCCAAG3610.11385642106059937No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3610.11385642106059937No Hit
GTCTGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3600.11354102931250908No Hit
TATTAACATTGACATAACTGACTACTAATTCCCTGGATGCTGGATCTTCC3570.11259485406823816No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC3570.11259485406823816No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3530.11133328707587695No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3520.11101789532778665No Hit
CCTTTACCCCGTTGCTCGGCAACGACCAGGTCTCTGCCAAGTGTTTGCTG3480.10975632833542542No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG3460.10912554483924483No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3450.10881015309115452No Hit
ACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC3450.10881015309115452No Hit
ATACAATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3430.10817936959497391No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGGTGGCC3420.10786397784688362No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3390.1069178026026127No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3340.1053408438621612No Hit
GGAAAATAGAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGAAATGT3320.10471006036598057No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3300.10407927686979998No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3270.10313310162552906No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3240.10218692638125818No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3220.10155614288507755No Hit
AGCGAGAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCAAGAA3220.10155614288507755No Hit
CTCTGGGATCTAGCAGAGCTTGGTGAAATGTTGTGGAGTTCCACTGCATG3210.10124075113698726No Hit
GGATTAAAGACAGGTACGGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3200.10092535938889696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)