FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358411
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA21730.6062871954264797No Hit
GGAAAATACAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT19680.5490902901975665No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16070.4483679351359193No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14680.40958564329777825No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13580.37889462097982485No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11890.33174205032769644No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11700.32644087374550446No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10950.30551517671053624No Hit
ATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC10740.29965598154074513No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10660.2974239071903485No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10630.2965868793089498No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10540.2940757956647536No Hit
GGAAGATACAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT10200.28458947967556797No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9570.2670118941661947No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9490.26477981981579807No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9400.2622687361716019No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9260.2583626060584078No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT9240.25780458747080864No Hit
ACTTCCAGGAACATCAACTACCAGCACGGGGCCATGCAAGACCTGCACGA9240.25780458747080864No Hit
CATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8430.23520483467304298No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT8330.23241474173504717No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8250.23018266738465057No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8140.22711356515285525No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7930.22125436998306414No Hit
GTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCTGCATT7930.22125436998306414No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7930.22125436998306414No Hit
CCTAGCAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAAC7750.21623220269467178No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7630.21288409116907683No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7590.21176805399387855No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7500.20925697034968235No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7460.20814093317448407No Hit
CTATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATA7360.20535084023648825No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACTG7330.20451381235508953No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7090.19781758930389973No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6600.18414613390772047No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6590.18386712461392088No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCA6510.18163505026352428No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATCCTTTGACATACT6470.18051901308832596No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCAAGAAGATTGACGATATGG6420.17912396661932808No Hit
GACGCAAATTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG6350.17717090156273108No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6240.17410179933093572No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6220.17354378074333657No Hit
ATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATATT6170.17214873427433866No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6120.17075368780534078No Hit
TATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTG6100.17019566921774165No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6040.16852161345494418No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG6000.16740557627974587No Hit
GTATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5860.16349944616655182No Hit
ATCTCAATGTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGG5800.16182539040375435No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC5610.1565242138215624No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACAAATCTT5580.15568718594016367No Hit
GATTTGGAACAGAAAGATTTGTCCCCATGCCTTGTCGAGGTTTGGAAGAC5220.14564285136337893No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5090.14201573054398442No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5090.14201573054398442No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5080.14173672125018486No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG5080.14173672125018486No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4970.1386676190183895No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4880.1361565353741933No Hit
TTCTCTATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCA4850.1353195074927946No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4770.133087433142398No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4720.1316923866734001No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4700.13113436808580095No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG4690.13085535879200136No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4650.12973932161680304No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTG4650.12973932161680304No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACTGCACTCAGGCA4630.1291813030292039No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4620.12890229373540432No Hit
GCATTCGACAAGGCATGGGGACAAATCTTTCTGTTCCCAATCCTCTGGGA4590.1280652658540056No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4540.12667021938500772No Hit
TCCTAGCAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA4530.12639121009120813No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4530.12639121009120813No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT4520.12611220079740856No Hit
GAAACTTAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA4510.12583319150360897No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4500.1255541822098094No Hit
GTCTCTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT4460.1244381450346111No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTAGAGGCAGGAGGAGGTGC4460.1244381450346111No Hit
GTCGAATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA4450.12415913574081153No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4440.12388012644701195No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4410.12304309856561323No Hit
TTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCTGCATTC4400.12276408927181365No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4380.1222060706842145No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4380.1222060706842145No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4330.1208110242152166No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4260.11885795915861957No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4180.11662588480822296No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG4150.11578885692682422No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAAACCTGGCCCGAATGCTCCCG4130.11523083833922508No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTGTGCCAAGTGTTTGCTG4070.11355678257642762No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4020.11216173610742973No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4020.11216173610742973No Hit
GGTCTTGCATGGCCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4010.11188272681363015No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3990.11132470822603101No Hit
CCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAAC3980.11104569893223143No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC3940.10992966175703313No Hit
GTTCCAAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCTGCATT3940.10992966175703313No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA3940.10992966175703313No Hit
GTCCAAGGTATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3910.1090926338756344No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3880.10825560599423567No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG3870.10797659670043608No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3860.1076975874066365No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3840.10713956881903736No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG3830.10686055952523778No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT3800.10602353164383906No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3760.10490749446864076No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3720.10379145729344245No Hit
ACTACACGCAGCGCATCATTTTGTGGGTCACCATATTCTTGGGAACAAGA3710.10351244799964288No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT3710.10351244799964288No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3690.1029544294120437No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3690.1029544294120437No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3680.10267542011824413No Hit
CCTTATGAGTCCAAGGTATACTAACATTGAGATTCCCGAGATTGAGATCT3660.10211740153064498No Hit
GTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGG3630.10128037364924626No Hit
TATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATG3610.1007223550616471No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA3600.10044334576784752No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3600.10044334576784752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)