FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358411
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA21390.596800879437294No Hit
GGAAAATACAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT18690.5214683701114083No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16040.4475309072545206No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15220.42465214516295535No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13360.3727564165162342No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11820.3297889852710994No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT11190.31221139976172607No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10940.30523616741673665No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10760.3002140001283443No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10150.2831944332065701No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9680.27008099639799No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9340.2605946804088044No Hit
GGAAGATACAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT9240.25780458747080864No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT9100.2538984573576146No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9080.25334043877001544No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA9070.25306142947621585No Hit
ACTTCCAGGAACATCAACTACCAGCACGGGGCCATGCAAGACCTGCACGA8960.2499923272444205No Hit
ATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC8850.24692322501262517No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8830.24636520642502602No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT8430.23520483467304298No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8410.23464681608544383No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8110.22627653727145652No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7940.2215333792768637No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7750.21623220269467178No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7740.21595319340087216No Hit
CATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7610.21232607258147768No Hit
GTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCTGCATT7300.2036767844736908No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7160.19977065436049676No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACTG7100.19809659859769932No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7060.19698056142250098No Hit
CTATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATA6870.19167938484030903No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATCCTTTGACATACT6820.19028433837131115No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6760.1886102826085137No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6600.18414613390772047No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6560.18303009673252216No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCAAGAAGATTGACGATATGG6320.17633387368133233No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6150.17159071568673953No Hit
GTATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6150.17159071568673953No Hit
TATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTG6140.17131170639293994No Hit
CCTAGCAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAAC6110.17047467851154122No Hit
ATCTCAATGTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGG6090.16991665992394206No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6060.16907963204254334No Hit
GACGCAAATTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG6010.16768458557354543No Hit
ATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATATT5770.16098836252235563No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG5660.15791926029056028No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5440.1517810558269696No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5280.14731690712617637No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5270.1470378978323768No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5270.1470378978323768No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5240.14620086995097809No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCA5210.14536384206957934No Hit
GATTTGGAACAGAAAGATTTGTCCCCATGCCTTGTCGAGGTTTGGAAGAC5160.14396879560058146No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5080.14173672125018486No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG5080.14173672125018486No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACAAATCTT5000.13950464689978823No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4990.13922563760598866No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4910.13699356325559206No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4770.133087433142398No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4750.13252941455479883No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4720.1316923866734001No Hit
GCATTCGACAAGGCATGGGGACAAATCTTTCTGTTCCCAATCCTCTGGGA4650.12973932161680304No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4650.12973932161680304No Hit
TTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCTGCATTC4560.12722823797260688No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACTGCACTCAGGCA4550.1269492286788073No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4470.12471715432841067No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4460.1244381450346111No Hit
GTCTCTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT4460.1244381450346111No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4450.12415913574081153No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4400.12276408927181365No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4330.1208110242152166No Hit
GTCGAATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA4290.1196949870400183No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG4250.11857894986482No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAAACCTGGCCCGAATGCTCCCG4220.11774192198342127No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4200.1171839033958221No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTGTGCCAAGTGTTTGCTG4190.11690489410202252No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTAGAGGCAGGAGGAGGTGC4170.11634687551442338No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4160.1160678662206238No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4130.11523083833922508No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4130.11523083833922508No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4070.11355678257642762No Hit
TTCTCTATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCA4070.11355678257642762No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT4070.11355678257642762No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4000.11160371751983059No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3990.11132470822603101No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT3990.11132470822603101No Hit
TCCTAGCAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA3980.11104569893223143No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA3950.11020867105083271No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3950.11020867105083271No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3920.10937164316943399No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3880.10825560599423567No Hit
GTCCAAGGTATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3870.10797659670043608No Hit
GAAACTTAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA3860.1076975874066365No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3860.1076975874066365No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3850.10741857811283693No Hit
GTTCCAAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCTGCATT3840.10713956881903736No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3820.1065815502314382No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3810.10630254093763863No Hit
GGGTTACTTTTGGAAGAGAAACTGTACTTGAGTATTTGGTGTCTTTTGGA3790.10574452235003948No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTG3730.10407046658724203No Hit
CCTTATGAGTCCAAGGTATACTAACATTGAGATTCCCGAGATTGAGATCT3700.10323343870584328No Hit
GGTCTTGCATGGCCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3690.1029544294120437No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3650.1018383922368454No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3640.10155938294304583No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA3640.10155938294304583No Hit
ACTACACGCAGCGCATCATTTTGTGGGTCACCATATTCTTGGGAACAAGA3640.10155938294304583No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3630.10128037364924626No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3590.10016433647404796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)