FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354891
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15940.4491519931471916No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11290.31812584709107866No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA11260.3172805171165231No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10840.30544589747274514No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10610.2989650343344858No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9140.25754386558126297No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8920.2513447791011888No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8880.25021767246844806No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8850.2493723424938925No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAGAGTCCAA8390.2364106162173738No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8200.2310568597118552No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA8160.22992975307911442No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7960.22429421991541063No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7940.22373066659904026No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7800.21978579338444762No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCATGGTGGTCTCCATGC7760.21865868675170685No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG7740.21809513343533649No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATACCCACATTGTGTAAAA7580.21358670690437348No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7530.21217782361344753No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7260.2045698538424474No Hit
CTATTAATAATGTCCTCCTGTAAGTGAATGTTAGGAAAGGAGGGAGTTTG6680.18822680766770644No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6630.18681792437678046No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTC6460.18202772118763227No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGTCAACTTACAAGGCC6370.17949173126396556No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6340.17864640128940998No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6010.16934777156929873No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5680.1600491418491875No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5670.15976736519100232No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5640.15892203521644674No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5550.15638604529278002No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5470.15413183202729852No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG5440.15328650205274297No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG5380.1515958421036318No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5280.14877807552177993No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA5240.14765096888903917No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGACGA5100.1437060956744465No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5040.1420154357253354No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5010.14117010575077982No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5000.14088832909259463No Hit
CTCCATGTTCGGTGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGG4960.13976122245985387No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4940.1391976691434835No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4930.1389158924852983No Hit
TTGTAAGAGGGCCCACATACTGTTGACATCTATTAATAATGTCCTCCTGT4910.13835233916892792No Hit
CATATAAAGGCATCAAGGCAGGATACCCACATTGTGTAAAAGGGGCAGCA4900.13807056251074273No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4870.13722523253618715No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4870.13722523253618715No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4870.13722523253618715No Hit
CTCCAAAAGACACCAAATACTCGAGAACAGTTTCTCTTCCAAAAGTAAGA4800.13525279592889083No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4780.13468924261252047No Hit
CTTTTACACAATGTGGGTATCCTGCCTTGATGCCTTTATATGCATGTATA4770.13440746595433525No Hit
GCCCTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCAAC4760.1341256892961501No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4710.13271680600522415No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAACCGGGCAAC4680.13187147603066857No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4660.1313079227142982No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT4650.131026146056113No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4610.12989903942337225No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4600.12961726276518706No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4590.12933548610700188No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG4590.12933548610700188No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4590.12933548610700188No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4560.1284901561324463No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4510.12708127284152035No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4500.12679949618333516No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4460.1256723895505944No Hit
GTATGTGGGCCCTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTA4440.12510883623422403No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4420.12454528291785366No Hit
CTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA4400.12398172960148326No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4360.12285462296874251No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA4350.12257284631055733No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGGAGTTCCACTGCATG4340.12229106965237213No Hit
GGATACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4320.12172751633600176No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4320.12172751633600176No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4310.12144573967781656No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4280.120600409703261No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCA4260.12003685638689063No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4170.11750086646322391No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCCCTCAGGCA4170.11750086646322391No Hit
TTATTAATAGATGTCAACAGTATGTGGGCCCTCTTACAATTAATGAAAAA4160.11721908980503873No Hit
ATGTGGGTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC4080.11496487653955721No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4070.11468309988137203No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4060.11440132322318684No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4020.11327421659044609No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4020.11327421659044609No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3980.11214710995770531No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3980.11214710995770531No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3970.11186533329952013No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3930.11073822666677938No Hit
CCTGTAAGTGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG3920.11045645000859418No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA3870.10904756671766824No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCAACCTGGGTGGGAAGTAATTTGG3830.10792046008492749No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC3810.10735690676855711No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATACCCACATTGTGT3800.10707513011037192No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3800.10707513011037192No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTAT3800.10707513011037192No Hit
GTGATTCGCAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGA3780.10651157679400153No Hit
GTTTACTAGTGCCATTTGTTCAGTGATTCGCAGGGCTTTCCCCCACTGTT3750.10566624681944596No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAATCACTGAACA3740.10538447016126078No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCACTTACAGG3740.10538447016126078No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG3710.10453914018670521No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTATTACCAATTTTCTT3710.10453914018670521No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3650.10284848023759408No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3640.10256670357940889No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3630.1022849269212237No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3620.10200315026303851No Hit
CCCACATACTGTTGACATCTATTAATAATGTCCTCCTGTAAGTGAATGTT3620.10200315026303851No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT3610.10172137360485332No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC3600.10143959694666814No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3600.10143959694666814No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3590.10115782028848293No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT3590.10115782028848293No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC3580.10087604363029776No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC3550.10003071365574218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)