FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580684
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25050.4313878116152675No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG19620.3378773997561496No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16490.28397544964214616No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15240.2624491117371927No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT11840.20389747263571925No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11660.20079767997740597No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT11220.19322040903486234No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA10940.18839850934415275No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG10630.1830599775437243No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT9870.16997196409751258No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9740.16773322495539741No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9720.1673888035489182No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA9650.16618332862624077No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA9580.16497785370356338No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9530.16411680018736524No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9340.16084479682581232No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9150.1575727934642594No Hit
ATTTCCGAGAGAGGACAACCGAGTTGTCAGTCCCGATGAGTTTCGCTCCA9120.1570561613545405No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8940.1539563686962272No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA8920.15361194728974795No Hit
GTGTCAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC8760.15085657603791391No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8760.15085657603791391No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAA8670.14930667970875725No Hit
AGTCTAGACTCCGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8520.14672351916016282No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC8370.1441403586115684No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG8060.13880182681113995No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7820.13466876993338892No Hit
CTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG7580.13053571305563783No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC7530.12967465953943969No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7480.12881360602324154No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7430.1279525525070434No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG7410.12760813110056418No Hit
ACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA7360.12674707758436604No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7300.12571381336492826No Hit
CTCACAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7250.12485275984873011No Hit
ATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCCCCTCACCATACAG7250.12485275984873011No Hit
CCCTCATAAAACCAAACGTTGGGGCTATTCCCTTAATTTCATGGGATATG7240.1246805491454905No Hit
ACACAAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA7150.12313065281633383No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAAACTCTACG6810.1172754889061865No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6730.11589780328026948No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6700.11538117117055059No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6660.11469232835759208No Hit
AACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT6530.11245358921547692No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC6450.1110759035895599No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGA6390.11004263937012214No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6350.10935379655716362No Hit
CCCACGTGTCGTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6280.10814832163448622No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC6180.10642621460208994No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG6180.10642621460208994No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC6180.10642621460208994No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6070.10453189686645405No Hit
AACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT6060.10435968616321442No Hit
CTCGAAAATACACCTCCTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGG6000.10332642194377664No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6000.10332642194377664No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5970.10280978983405777No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5960.10263757913081814No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG5930.10212094702109925No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5910.10177652561462No Hit
CCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGC5860.10091547209842187No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5850.10074326139518222No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5830.10039883998870298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)