FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384146
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG13720.35715587302744267No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11880.30925741775262533No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11450.29806375700905385No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10940.2847875547317947No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10880.2832256485815289No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA10190.26526372785347235No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9410.24495894790001718No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9230.24027322944921983No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT9180.23897164099066498No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG8960.2332446517730238No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8860.23064147485591413No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8690.22621607409682776No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8580.22335257948800716No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG8550.22257162641287428No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC8310.21632400181181116No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACATTGCTGAGAGTCCAA8290.2158033664283892No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8150.2121589187444357No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8020.20877478875219319No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7880.20513034106823969No Hit
CTGTAAGAGGGCCCACGTATTGTTGACATCTATTAATAATGTCCTCCTGT7530.1960192218583559No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6980.1817017488142529No Hit
TTATTAATAGATGTCAACAATACGTGGGCCCTCTTACAGTTAATGAAAAA6780.17649539498003364No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6680.17389221806292401No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6350.1653017342364622No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5990.15593029733486746No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5940.15462870887631266No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5830.15176521426749204No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTTCACCAAGCTCTGCTAG5760.1499429904255153No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5760.1499429904255153No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5690.14812076658353857No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5480.14265409505760832No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5470.14239377736589734No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGAAATGTGGTGG5460.1421334596741864No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT5450.14187314198247541No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5440.14161282429076444No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5380.14005091814049866No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5360.13953028275707674No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5300.13796837660681097No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5290.1377080589151No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA5280.13744774122338904No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5250.1366667881482561No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG5150.1340636112311465No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5070.1319810696974588No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5020.13067948123890394No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5020.13067948123890394No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5000.130158845855482No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5000.130158845855482No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4980.12963821047206012No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4940.12859693970521624No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4940.12859693970521624No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4930.12833662201350526No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC4920.12807630432179432No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC4900.1275556689383724No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4890.12729535124666144No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4830.12573344509639564No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4820.1254731274046847No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4780.12443185663784083No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4680.12182867972073118No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4660.12130804433730924No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4620.1202667735704654No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4610.12000645587875443No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4610.12000645587875443No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4580.11922550280362154No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4570.11896518511191057No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4500.11714296126993382No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4500.11714296126993382No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4470.11636200819480093No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4430.11532073742795707No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4360.11349851358598033No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4330.11271756051084744No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC4260.11089533666887069No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4250.11063501897715972No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4220.10985406590202683No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4220.10985406590202683No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4210.10959374821031588No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCCGGATAATAAGGTTTAATGCC4170.10855247744347202No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4170.10855247744347202No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4160.10829215975176104No Hit
GCCATGGGAATGAGGTGTATTTCCGAGAGAGGACAACCGAGTTGTCAGTC4140.10777152436833913No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4130.10751120667662815No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4120.1072508889849172No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC4120.1072508889849172No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4080.10620961821807334No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4070.10594930052636237No Hit
GAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAATACTAAC4070.10594930052636237No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4030.10490802975951852No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4030.10490802975951852No Hit
GGGTTCACCCCACCACACGGCGGTATTTTGGGGTGGAGCCCTCAGGCTCA4020.10464771206780754No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4010.10438739437609659No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACA3930.10230485284240887No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3920.10204453515069792No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT3920.10204453515069792No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC3910.10178421745898694No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3890.10126358207556503No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG3890.10126358207556503No Hit
CTCTGGGATCTAGCAGAGCTTGGTGAAATGTGGTGGAGTTCCACTGCATG3880.10100326438385406No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3880.10100326438385406No Hit
ATTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC3870.10074294669214309No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3850.10022231130872117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)