FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459019
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA21790.47470801862232287No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT18750.4084798232752892No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17280.37645500513050656No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT16880.36774076890063373No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA16540.36033366810524187No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGTTCACCCGTG16240.3537979909328372No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATCACATACCCCATGAA15570.3392016452478002No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG15480.3372409420960788No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG15440.33636951847309154No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA15320.33375524760412967No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG14050.3060875475742834No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC12610.27471629714674123No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC12410.2703591790318048No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAGGGAGTTT11850.2581592483099828No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG11230.24465218215367993No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTATCA11150.24290933490770536No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTATGTTGTCGAGAAAGTAA11080.2413843435674776No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA10790.23506652230081979No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTTCACCAAGCTCTGCAAG10570.23027369237438974No Hit
CCATTTACACAATGTGGTTACCCTGCTTTAATGCCTTTGTATGCATGTAT10350.22548086244795965No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTGTT10170.2215594561445169No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGGTAACCACATTGTGTAAAT9960.21698448212383367No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC9800.2134987876318845No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT9770.21284521991464406No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCAAGGTGGTCTCCATGC9390.20456669549626488No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT9340.20347741596753077No Hit
CTACTAGACACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA9160.19955600966408799No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC8910.19410961202041746No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8840.19258462068018972No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTAAATTTCAT8840.19258462068018972No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAATTCCACAGCTTT8440.18387038445031686No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA8420.18343467263882324No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAG8060.17559186003193766No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT7860.17123474191700128No Hit
CAGCACTAAATATTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTG7840.17079903010550762No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7770.16927403876527986No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT7590.16535263246183707No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7560.16469906474459664No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA7530.16404549702735619No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT7530.16404549702735619No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA7350.1601240907239134No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT7340.15990623481816657No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC7300.1590348111951793No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7240.15772767576069835No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC7150.15576697260897698No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7090.15445983717449605No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7090.15445983717449605No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG7080.15424198126874925No Hit
GTCTAGAGGCAAATATTTAGTGCTGTTAGGATAAAACCTAGCAGGCATAA7060.1538062694572556No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAGGGAGTTTGCCATTCAGGAT7030.1531527017400151No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT7010.15271698992852148No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6980.15206342221128102No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGGGAGATGGGAGTAGGCTGCCT6950.15140985449404054No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG6880.1498848631538128No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT6840.14901343953082552No Hit
TTCTTCTACTGTTCCTATCTTTAATCCTGAATGGCAAACTCCCTCTTTTC6810.14835987181358506No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6800.14814201590783824No Hit
GGGTTGAGTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGGCTCCT6750.14705273637910413No Hit
ATTTGGAACAGGGTTGAGTGTTCCTGAACTGGAGCCACCAGCAGGAAAAT6720.14639916866186367No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAAAATTCATTTACATG6720.14639916866186367No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG6650.1448741773216359No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6650.1448741773216359No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC6630.1444384655101423No Hit
ATTTAGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTC6610.14400275369864865No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT6520.14204205054692726No Hit
ATTTACATACTCTTTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACA6500.1416063387354336No Hit
ATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGACTCTA6340.13812064424348447No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA6260.1363777969975099No Hit
CCCATGATGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGG6190.13485280565728217No Hit
CTCTTAGAGGTGGGGAGATGGGAGTAGGCTGCCTTCCTGACTGCCGATTG6190.13485280565728217No Hit
GTCCAACTGATGATCGGGAAAAAATCCCAGAGGGTTGGGAACAGAAAGAT6170.1344170938457885No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTATGTTGTCGA6150.13398138203429488No Hit
CTCTTTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACACGTAGCGCC6050.13180282297682666No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT6030.13136711116533303No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG6000.13071354344809258No Hit
GGATTAAAGATAGGAACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC5950.12962426391935844No Hit
TTCCCCCACTGTTTGGCTTTCAGCTATATGGATGATGTGGTACTGGGGGC5910.12875284029637116No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5880.12809927257913073No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG5870.12788141667338387No Hit
GTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCGAGGACTGGGGA5780.1259207135216625No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGACAACATACAAGGCC5740.12504928989867523No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGAC5630.1226528749354602No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5620.12243501902971336No Hit
GAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAATACTAAC5540.1206921717837388No Hit
GTACAGACTTGGCCCCCAGTACCACATCATCCATATAGCTGAAAGCCAAA5530.12047431587799198No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTATGTTGTCGAGAAAGTA5490.1196028922550047No Hit
GTACGGCAGACGGAGAAGGGGACGATAGAGTCCCAAGCGGCCCCGCGAGG5470.11916718044351106No Hit
CAGTTGGACCCTGCATTCGGAGCCAATTCAAACAATCCAGATTGGGACTT5460.11894932453776423No Hit
GTTTTAGAAAACTTCCTATCAATCGCCCTATTGATTGGAAAGTATGTCAG5450.11873146863201742No Hit
GCACTAAATATTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGAT5440.11851361272627059No Hit
ACCTAGCAGCCATGGAAAGGATGTATATTTCCGAGAAAGGACGACAGAAT5440.11851361272627059No Hit
CTATTGTGGTTTCATATTTCTTGCCTTACTTTTGGAAGAGAAACTGTCCT5400.1176421891032833No Hit
GTTCAGGAACACTCAACCCTGTTCCAAATATTGCCTCTCACATCTCGTCA5390.11742433319753649No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA5380.11720647729178965No Hit
GGATGTATATTTCCGAGAAAGGACGACAGAATTATCAGTCCCGATGAGTT5360.11677076548029601No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA5340.11633505366880237No Hit
ACTAGACACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC5340.11633505366880237No Hit
GGACTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTTGCCTTACTTT5330.11611719776305557No Hit
CTCCTACCTGGTTGGCTTGTGGCCAGTGGTCCTTGATGGGGTTGAAGTCC5270.11481006232857463No Hit
GCTTTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT5270.11481006232857463No Hit
CCCCAAAACACCTCCGTGTGGGGGAGTGAACCCTGGCCCGAATGCTCCCA5270.11481006232857463No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5250.11437435051708099No Hit
TATACATCCTTTCCATGGCTGCTAGGTTGTACTGCCAACTGGATTCTTCG5210.1135029268940937No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA5170.11263150327110642No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA5160.11241364736535961No Hit
GTCCTTGAGTATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5090.11088865602513186No Hit
TCACCATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGA5080.11067080011938504No Hit
GTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCAAGCCATTC5060.1102350883078914No Hit
CCCCCACACGGAGGTGTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATT5060.1102350883078914No Hit
GGGTTCACTCCCCCACACGGAGGTGTTTTGGGGTGGAGCCCTCAGGCTCA5040.10979937649639776No Hit
CACATACCCCATGAAATTTAGGGAATAACCCCATCTCTTTGTTTTGTTAG4990.10871009696766365No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCAGGGTCCCGTA4960.1080565292504232No Hit
CAATAGGGCGATTGATAGGAAGTTTTCTAAAACAGTGTTTGATTTTTTGT4930.10740296153318271No Hit
GTGCCAGCAGTTCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCA4850.10566011428720815No Hit
ATGTTAATACTAACATGGGACTAAAGATCAGGCAACTATTGTGGTTTCAT4830.1052244024757145No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATATACTGTTTACTT4810.10478869066422086No Hit
CCATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGACTC4800.10457083475847405No Hit
GAATATGGTGACCCGCAAAATGAGGCGCTACGTGTAGTTTCCCTCTTATA4800.10457083475847405No Hit
GTTCCTATCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAAAATTCA4780.10413512294698041No Hit
GCTTTATTCTTCTACTGTTCCTATCTTTAATCCTGAATGGCAAACTCCCT4760.10369941113548677No Hit
TTCCTGAACTGGAGCCACCAGCAGGAAAATACAGGCTCCTGACTCTGGGA4760.10369941113548677No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCATCT4740.10326369932399312No Hit
CTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGACCGAGGCA4730.10304584341824631No Hit
ATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGACCGAGG4710.10261013160675267No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTTCCTATC4710.10261013160675267No Hit
GAATTATCAGTCCCGATGAGTTTTGCTCCAGACCGGCTGCGAGCAAAACA4700.10239227570100584No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4700.10239227570100584No Hit
ATATGGATGATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCC4660.10152085207801856No Hit
CCATATTCTTGGGAACAAGAGCTACATCATGGGAGGTTGGTCATCAAAAC4640.10108514026652492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)