FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459019
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA29010.6319999825715276No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT17450.38015855552820255No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG17000.3703550397695956No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGTTCACCCGTG16490.35924438857650776No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATCACATACCCCATGAA15870.34573732242020483No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG15780.3437766192684834No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT15560.33898378934205337No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA15100.3289624176776996No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15080.32852670586620597No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14730.3209017491650672No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG14110.3073946830087643No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC12240.26665562863410885No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTATGTTGTCGAGAAAGTAA11370.2477021648341354No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTATCA11290.24595931758816084No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAGGGAGTTT11090.2416021994732244No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTGTT10950.23855221679276892No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC10880.23702722545254118No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTTCACCAAGCTCTGCAAG10390.22635228607094693No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC10300.22439158291922556No Hit
CCATTTACACAATGTGGTTACCCTGCTTTAATGCCTTTGTATGCATGTAT10040.21872732936980824No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGGTAACCACATTGTGTAAAT9830.214152355349125No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA9810.21371664353763134No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG9770.21284521991464406No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT9770.21284521991464406No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC9680.21088451676292266No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA8880.193456044303177No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT8800.19171319705720244No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8740.1904060616227215No Hit
CTACTAGACACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA8640.18822750256525328No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAG8640.18822750256525328No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAATTCCACAGCTTT8600.187356078942266No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTAAATTTCAT8400.18299896082732958No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCAAGGTGGTCTCCATGC7970.1736311568802163No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG7760.16905618285953306No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT7750.16883832695378623No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT7660.16687762380206483No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7530.16404549702735619No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC7490.1631740734043689No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC7480.16295621749862205No Hit
TTCTTCTACTGTTCCTATCTTTAATCCTGAATGGCAAACTCCCTCTTTTC7320.15947052300667292No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7310.15925266710092612No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA7240.15772767576069835No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA7240.15772767576069835No Hit
CAGCACTAAATATTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTG7230.15750981985495155No Hit
GTCTAGAGGCAAATATTTAGTGCTGTTAGGATAAAACCTAGCAGGCATAA7210.1570741080434579No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAGGGAGTTTGCCATTCAGGAT7180.1564205403262174No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGGGAGATGGGAGTAGGCTGCCT7080.15424198126874925No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT7080.15424198126874925No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG7030.1531527017400151No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT7010.15271698992852148No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6960.15162771039978737No Hit
ATTTGGAACAGGGTTGAGTGTTCCTGAACTGGAGCCACCAGCAGGAAAAT6880.1498848631538128No Hit
CTCTTAGAGGTGGGGAGATGGGAGTAGGCTGCCTTCCTGACTGCCGATTG6820.1485777277193319No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT6800.14814201590783824No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG6800.14814201590783824No Hit
ATTTAGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTC6720.14639916866186367No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6630.1444384655101423No Hit
GGGTTGAGTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGGCTCCT6580.14334918598140817No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTATGTTGTCGA6580.14334918598140817No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6570.14313133007566137No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAAAATTCATTTACATG6500.1416063387354336No Hit
GTACAGACTTGGCCCCCAGTACCACATCATCCATATAGCTGAAAGCCAAA6480.14117062692393997No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6430.14008134739520586No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT6410.13964563558371224No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6280.13681350880900353No Hit
GTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTACCCCAACTTC6250.13615994109176308No Hit
GTCCAACTGATGATCGGGAAAAAATCCCAGAGGGTTGGGAACAGAAAGAT6210.1352885174687758No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTATGTTGTCGAGAAAGTA6050.13180282297682666No Hit
GAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAATACTAAC6040.13158496707107986No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG6010.13093139935383938No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5940.12940640801361164No Hit
CTCTTTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACACGTAGCGCC5940.12940640801361164No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA5920.128970696202118No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5870.12788141667338387No Hit
GGATTAAAGATAGGAACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC5860.12766356076763707No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5800.12635642533315614No Hit
GTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCGAGGACTGGGGA5750.12526714580442203No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5750.12526714580442203No Hit
GTTTTAGAAAACTTCCTATCAATCGCCCTATTGATTGGAAAGTATGTCAG5720.12461357808718157No Hit
CCCATGATGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGG5700.12417786627568793No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGAC5690.1239600103699411No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5630.1226528749354602No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATATACTGTTTACTT5620.12243501902971336No Hit
CAGTTGGACCCTGCATTCGGAGCCAATTCAAACAATCCAGATTGGGACTT5610.12221716312396655No Hit
GTTCAGGAACACTCAACCCTGTTCCAAATATTGCCTCTCACATCTCGTCA5610.12221716312396655No Hit
ATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGACTCTA5600.12199930721821972No Hit
GTACGGCAGACGGAGAAGGGGACGATAGAGTCCCAAGCGGCCCCGCGAGG5580.12156359540672608No Hit
ATTTACATACTCTTTGGAAGGCTGGGATTCTATATAAGAGGGAAACTACA5510.12003860406649834No Hit
TTCCCCCACTGTTTGGCTTTCAGCTATATGGATGATGTGGTACTGGGGGC5490.1196028922550047No Hit
GCTTTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT5490.1196028922550047No Hit
GGACTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTTGCCTTACTTT5490.1196028922550047No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA5430.11829575682052378No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5380.11720647729178965No Hit
GGATGTATATTTCCGAGAAAGGACGACAGAATTATCAGTCCCGATGAGTT5340.11633505366880237No Hit
GGGTTCACTCCCCCACACGGAGGTGTTTTGGGGTGGAGCCCTCAGGCTCA5200.11328507098834689No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT5160.11241364736535961No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGACAACATACAAGGCC5120.11154222374237233No Hit
GTCCTTGAGTATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5120.11154222374237233No Hit
CTATTGTGGTTTCATATTTCTTGCCTTACTTTTGGAAGAGAAACTGTCCT5100.11110651193087868No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA5070.11045294421363822No Hit
GTTCCTATCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAAAATTCA5060.1102350883078914No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5040.10979937649639776No Hit
ACCTAGCAGCCATGGAAAGGATGTATATTTCCGAGAAAGGACGACAGAAT5040.10979937649639776No Hit
TATACATCCTTTCCATGGCTGCTAGGTTGTACTGCCAACTGGATTCTTCG5030.10958152059065093No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT5020.10936366468490412No Hit
GTGCCAGCAGTTCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCA4970.10827438515617No Hit
GCACTAAATATTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGAT4940.10762081743892955No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA4920.10718510562743591No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCAGGGTCCCGTA4890.10653153791019543No Hit
CAATAGGGCGATTGATAGGAAGTTTTCTAAAACAGTGTTTGATTTTTTGT4890.10653153791019543No Hit
CTTTAGTCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG4890.10653153791019543No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA4840.10544225838146133No Hit
CCCCCACACGGAGGTGTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATT4820.10500654656996769No Hit
CTCCTACCTGGTTGGCTTGTGGCCAGTGGTCCTTGATGGGGTTGAAGTCC4820.10500654656996769No Hit
GAATTATCAGTCCCGATGAGTTTTGCTCCAGACCGGCTGCGAGCAAAACA4810.10478869066422086No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA4800.10457083475847405No Hit
GCCTACTCCCATCTCCCCACCTCTAAGAGACAGTCATCCTCAGGCCATGC4780.10413512294698041No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCATCT4760.10369941113548677No Hit
CACATACCCCATGAAATTTAGGGAATAACCCCATCTCTTTGTTTTGTTAG4760.10369941113548677No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTTCCTATC4690.10217441979525903No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4680.1019565638895122No Hit
ACTAGACACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC4660.10152085207801856No Hit
GAATATGGTGACCCGCAAAATGAGGCGCTACGTGTAGTTTCCCTCTTATA4660.10152085207801856No Hit
ATGATGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC4630.1008672843607781No Hit
CTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAATTCCACAGCTTTTCA4610.10043157254928446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)