FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347056
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15480.44603752708496613No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12020.3463418007468535No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11400.3284772486284634No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11270.3247314554423493No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11180.32213821400580883No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG10390.29937531695173114No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8430.24290028122262688No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8270.23829007422433265No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8230.2371375224747591No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA8160.23512055691300537No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8060.23223917753907153No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7630.2198492462311558No Hit
TTGTACACCTCACCATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTG7590.21869669448158222No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7590.21869669448158222No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA7400.21322207367110782No Hit
CTTTAGGGCATGGACATTGACCTGTATAAAGAATTTGGAGCATCTGTGGA7380.21264579779632103No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7290.21005255635978054No Hit
CCCTTGGACGCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7090.2042897976119128No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6870.19795076298925823No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6830.19679821123968466No Hit
TTGTTCACCTCACCATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTG6700.1930524180535706No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACACAAGAAAATTGGTAATAGA6690.19276428011617722No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6580.18959476280484994No Hit
GTGTAAAAGGGGCAGCAAAGCCTAAAAGGCCCACAATTCTTTGACATACT6280.18095062468304826No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT5940.17115393481167304No Hit
GTATCGTTGACATCTATCAATAATGTCCTCCTGTAAATGAATGTGAGGAA5640.16250979668987137No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG5450.157035175879397No Hit
ATATCGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCA5400.15559448619243005No Hit
CAACAATACGTCGGCCCTCTTACAATTAATGAAAAAAGGAGATTAAAATT5390.15530634825503667No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5200.14983172744456225No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5170.14896731363238208No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5050.14550965838366142No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATG4910.14147572726015398No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4890.14089945138536722No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4870.14032317551058043No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4840.13945876169840027No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4750.13686552026185975No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4680.13484855470010604No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4670.13456041676271263No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4660.13427227882531925No Hit
CTTTGGGGCATGGACATTGACCTGTATAAAGAATTTGGAGCATCTGTGGA4570.13167903738877876No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATACC4560.13139089945138535No Hit
GGTGTACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAGAGCAGTGGC4560.13139089945138535No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4420.12735696832787793No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG4320.12447558895394403No Hit
GTATAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAATACCACATC4300.12389931307915725No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4240.12217048545479692No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4200.12101793370522336No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4180.12044165783043657No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4130.11900096814346964No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT4110.11842469226868285No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4040.11640772670692914No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4020.11583145083214237No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4020.11583145083214237No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4010.11554331289474898No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT3960.11410262320778203No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3960.11410262320778203No Hit
TTATTAATAGATGTCAACAATACGTCGGCCCTCTTACAATTAATGAAAAA3950.11381448527038865No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG3920.11295007145820848No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3900.1123737955834217No Hit
ACCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGC3890.1120856576460283No Hit
GCTTTAGGGCATGGACATTGACCTGTATAAAGAATTTGGAGCATCTGTGG3830.11035683002166798No Hit
TCACCATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTGAGTTGATGA3690.10632289889816052No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG3640.10488220921119357No Hit
GTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTTGTGTC3640.10488220921119357No Hit
GTATTAAAGCTCAAACAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT3640.10488220921119357No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3640.10488220921119357No Hit
GGTATAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAATACCACAT3620.1043059333364068No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3600.10372965746162002No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3590.10344151952422664No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3560.10257710571204648No Hit
CCATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTGAGTTGATGAATT3550.10228896777465309No Hit
ATCCTAACTTGACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT3530.10171269189986631No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3490.10056014015029276No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3480.10027200221289936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)