FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347056
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14200.40915587109861234No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13210.3806302152966668No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11180.32213821400580883No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10830.31205338619704026No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10650.3068669033239593No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG10440.30081600663869806No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9820.28295145452030795No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9540.27488359227329306No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG9060.26105297127841043No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8680.25010372965746164No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA8010.23079848785210458No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7720.22244248766769628No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7710.2221543497303029No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA7470.21523903923286156No Hit
CTTTAGGGCATGGACATTGACCTGTATAAAGAATTTGGAGCATCTGTGGA7450.21466276335807477No Hit
TTGTACACCTCACCATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTG7220.2080355907980268No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7090.2042897976119128No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7070.203713521737126No Hit
CCCTTGGACGCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6720.19362869392835738No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6700.1930524180535706No Hit
GTGTAAAAGGGGCAGCAAAGCCTAAAAGGCCCACAATTCTTTGACATACT6420.18498455580655573No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACACAAGAAAATTGGTAATAGA6380.18383200405698216No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6350.182967590244802No Hit
TTGTTCACCTCACCATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTG6320.18210317643262183No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT5790.16683186575077222No Hit
ATATCGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCA5390.15530634825503667No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5380.15501821031764326No Hit
GTATCGTTGACATCTATCAATAATGTCCTCCTGTAAATGAATGTGAGGAA5210.15011986538195565No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5180.1492554515697755No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5140.14810289982020192No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG5120.14752662394541513No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5080.1463740721958416No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4950.14262827900972755No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4840.13945876169840027No Hit
CAACAATACGTCGGCCCTCTTACAATTAATGAAAAAAGGAGATTAAAATT4800.1383062099488267No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4770.13744179613664653No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4720.13600110644967958No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4620.13311972707574568No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATG4600.13254345120095892No Hit
CTTTGGGGCATGGACATTGACCTGTATAAAGAATTTGGAGCATCTGTGGA4590.13225531326356552No Hit
TTATTAATAGATGTCAACAATACGTCGGCCCTCTTACAATTAATGAAAAA4400.12678069245309115No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4360.12562814070351758No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4330.12476372689133743No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATACC4250.12245862339219031No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4240.12217048545479692No Hit
GTATAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAATACCACATC4230.12188234751740352No Hit
GGTATAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAATACCACAT4190.12072979576782997No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4180.12044165783043657No Hit
GGTGTACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAGAGCAGTGGC4150.11957724401825641No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG4150.11957724401825641No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4120.11871283020607624No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4100.11813655433128946No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4050.11669586464432254No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4010.11554331289474898No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT4000.1152551749573556No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3870.11150938177124153No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3840.11064496795906137No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3840.11064496795906137No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3830.11035683002166798No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3720.10718731271034068No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGTTTGAGCTTTAATACAATA3710.1068991747729473No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG3650.10517034714858696No Hit
ATCGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3590.10344151952422664No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3580.10315338158683326No Hit
ACCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGC3570.10286524364943987No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3570.10286524364943987No Hit
GTATTAAAGCTCAAACAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT3570.10286524364943987No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3560.10257710571204648No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT3550.10228896777465309No Hit
CCTTTATACCTCTATTACCAATTTTCTTGTGTCTTTGGGTATACATTTAA3520.10142455396247292No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3490.10056014015029276No Hit
ATCCTAACTTGACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT3490.10056014015029276No Hit
TCACCATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTGAGTTGATGA3490.10056014015029276No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3480.10027200221289936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)