FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524856
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC62511.1909933391253982No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC29860.5689179508284177No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC23480.4473607999146432No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA19170.36524303809044767No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC17340.33037633179386344No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT15780.3006538936393982No Hit
GTCTAGAAGATCTCGTACGGAAGGAAAAAAGTCAGAAGGCAAAAACGAGA15630.2977959668937766No Hit
CCCCCACCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA15570.2966527961955279No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCGCTAAATTTCAT15570.2966527961955279No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15330.29208011340253326No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA14730.2806484064200466No Hit
GGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA14370.27378938223055466No Hit
GTAGCAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGGAT14200.2705503985855168No Hit
CTCTAATGCATCCCGATACAGAGCCGCGGCGGTGTCTAGAAGATCTCGTA13530.25778499245507336No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC13370.2547365372597436No Hit
CTCTATGGAAGGCGGGTGTTTTATATAAGAGAGTATCAACACATAGCGCC12570.2394942612830948No Hit
TTCTACTACTGTACCTGTCTTTAACCCTCATTGGAAAACGCCCTCTTTTC12230.23301629399301904No Hit
ACCCTTCTCGGGGCCGCTTGGGGCCCTGTCGTCCCCTTCTCTGCCTGCCG12100.2305394241468136No Hit
CTTCTAGACACCGCCGCGGCTCTGTATCGGGATGCATTAGAGTCTCCTGA11880.22634779825323517No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA11800.22482357065557027No Hit
CATTTGAACCCTAACAAAACAAAAAGATGGGGTTATTCGCTAAATTTCAT11450.21815507491578642No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC11410.21739296111695397No Hit
TCCCGATACAGAGCCGCGGCGGTGTCTAGAAGATCTCGTACGGAAGGAAA10550.2010075144420565No Hit
ATATAAGAGAGTATCAACACATAGCGCCTCATTTTGTGGGTCACCATATT10230.19491060405139696No Hit
GCATTAGAGTCTCCTGAGCATGTTTCAGCTCATCATACTGCACTCAGGCA10170.1937674333531483No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCCGTACG9730.18538418156599143No Hit
GCATAGCCTGAGGATGAGTGGTTCTCAGAGGTGGAGACAGCGGGGTAGGC9480.18062097032328867No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG9200.1752861737314616No Hit
ACCCGTCTCGGGGCCGCTTGGGGCCCTGTCGTCCCCTTCTCTGCCTGCCG9170.17471458838233725No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT9140.17414300303321292No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAACCCTAGCCCGAATGCTCCAG8960.17071349093846694No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACCTTGA8860.1688082064413858No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG8860.1688082064413858No Hit
CTATTTACACACTCTATGGAAGGCGGGTGTTTTATATAAGAGAGTATCAA8760.16690292194430473No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC8690.16556922279634795No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8570.16328288139985062No Hit
CCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC8510.16213971070160196No Hit
GAGACGGGTCGTCCGCAGGATTCAGCGCCGACGGGACGTAAACAAAGGAC8440.16080601155364518No Hit
GTATCGGGATGCATTAGAGTCTCCTGAGCATGTTTCAGCTCATCATACTG8210.15642385721035865No Hit
GGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTC8170.15566174341152622No Hit
AGATTGAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCG8160.1554712149618181No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8160.1554712149618181No Hit
ATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACCTG7990.15223223131678024No Hit
GCTTTAGGGCATGGACCTTGACCCTTATAAAGAATTTGGAGCTTCTGTGG7980.1520417028670721No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT7940.15127958906823968No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT7940.15127958906823968No Hit
CTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCT7920.15089853216882346No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT7850.14956483302086668No Hit
GGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA7690.1465163778255369No Hit
CTCTTATATAAAACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT7620.14518267867758014No Hit
ATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCGCTAAATTTCATG7500.1428963372810828No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7470.14232475193195848No Hit
GGGAAACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTC7390.1408005243342936No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7310.13927629673662872No Hit
CTCTAGAACCGTTTCTCTTCCAAAAGTGAGACAAGAAACGTGAAACCACA7290.1388952398372125No Hit
GGTTCAGGTACTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTG7230.13775206913896385No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG6990.13317938634596918No Hit
GAGAAGGGTCGTCCGCAGGATTCAGCGCCGACGGGACGTAAACAAAGGAC6980.13298885789626108No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6900.13146463029859617No Hit
TCTCCATCTCGGGGATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGG6890.13127410184888808No Hit
CCTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATAATACAAACCTTGCCAG6820.1299404027009313No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6790.12936881735180697No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG6760.12879723200268264No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTGTCAGTTCCGATAATGTTT6750.12860670355297452No Hit
GTTAATAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTCT6590.12555824835764476No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCCCCACC6580.12536771990793666No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6580.12536771990793666No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT6540.1246056061091042No Hit
CCCCAGAAGGCCTCCGTGCGGTGGGGTGAACCCTAGCCCGAATGCTCCAG6540.1246056061091042No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6540.1246056061091042No Hit
CTATAAGGTGGGGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC6530.1244150776593961No Hit
CTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTC6460.12308137851143933No Hit
CTCCAAAAGACACCAAATACTCTAGAACCGTTTCTCTTCCAAAAGTGAGA6340.120795037114942No Hit
ATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCA6300.12003292331610957No Hit
ATGTTTCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACCTG6280.11965186641669334No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6180.11774658191961224No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGT5970.11374548447574191No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5860.11164967152895271No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACCTTGACCCTTAT5800.11050650083070405No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCTTA5770.1099349154815797No Hit
ACCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGAGACTT5720.10898227323303915No Hit
CCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGAGACTTG5710.10879174478333105No Hit
GTAGTAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGGAT5680.10822015943420672No Hit
GTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGG5640.10745804563537427No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5580.10631487493712562No Hit
GTGTCTGATGTGATCTTGTGGCAATGATCCCCAACATCCAATGACATAGC5540.10555276113829316No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5490.10460011888975262No Hit
AGGTTCAGGTACTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGT5480.1044095904400445No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACC5470.1042190619903364No Hit
GTGCAGTATGATGAGCTGAAACATGCTCAGGAGACTCTAATGCATCCCGA5470.1042190619903364No Hit
GTGTAAACAGTACCTGAACCTTTACCCCGTTGCCAGGCAACGGCCAGGTC5460.10402853354062827No Hit
CTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATAATACAAACCTTGCCAGC5430.10345694819150394No Hit
GCATTAACGCAGGATAACCACATTGTGTGAAAGGGGCAGCAAAACCCAGA5430.10345694819150394No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCAGCAAAGAATTGCT5430.10345694819150394No Hit
GTTTAATACCCTTATCCAAGGGCAAATATTTGGTAAAATTAGGATAAAAC5410.10307589129208772No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5410.10307589129208772No Hit
CATTAACGCAGGATAACCACATTGTGTGAAAGGGGCAGCAAAACCCAGAA5380.10250430594296339No Hit
CTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTTTTGCCTTCT5380.10250430594296339No Hit
GTATCAACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACA5370.10231377749325529No Hit
CTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGAC5370.10231377749325529No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5360.10212324904354719No Hit
CTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCT5360.10212324904354719No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC5310.10117060679500664No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5270.10040849299617419No Hit
ATACAGAGCCGCGGCGGTGTCTAGAAGATCTCGTACGGAAGGAAAAAAGT5260.10021796454646609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)