FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524856
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC54411.0366652948618287No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC33050.6296965262853049No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC27490.5237627082475955No Hit
CCCCCACCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA16170.30808450317801456No Hit
ACCCTTCTCGGGGCCGCTTGGGGCCCTGTCGTCCCCTTCTCTGCCTGCCG15750.3000823082902739No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15290.2913179996037008No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCGCTAAATTTCAT15140.2884600728580792No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT15090.2875074306095386No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA15010.28598320301187374No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA14170.2699788132363925No Hit
GTCTAGAAGATCTCGTACGGAAGGAAAAAAGTCAGAAGGCAAAAACGAGA13230.25206913896383004No Hit
GGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA12640.2408279604310516No Hit
GTAGCAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGGAT12520.23854161903455423No Hit
CTTCTAGACACCGCCGCGGCTCTGTATCGGGATGCATTAGAGTCTCCTGA11730.22348987150761354No Hit
ACCCGTCTCGGGGCCGCTTGGGGCCCTGTCGTCCCCTTCTCTGCCTGCCG11720.22329934305790541No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC11360.21644031886841344No Hit
TTCTACTACTGTACCTGTCTTTAACCCTCATTGGAAAACGCCCTCTTTTC11030.21015288002804577No Hit
CTCTATGGAAGGCGGGTGTTTTATATAAGAGAGTATCAACACATAGCGCC10990.20939076622921332No Hit
CTCTAATGCATCCCGATACAGAGCCGCGGCGGTGTCTAGAAGATCTCGTA10850.20672336793329982No Hit
CATTTGAACCCTAACAAAACAAAAAGATGGGGTTATTCGCTAAATTTCAT10840.2065328394835917No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC10230.19491060405139696No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG10080.1920526773057753No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC9630.18347889706891032No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAACCCTAGCCCGAATGCTCCAG9580.18252625482036977No Hit
GCATTAGAGTCTCCTGAGCATGTTTCAGCTCATCATACTGCACTCAGGCA9560.18214519792095354No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG9470.18043044187358057No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACCTTGA9370.17852515737649946No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCCGTACG9350.17814410047708323No Hit
ATATAAGAGAGTATCAACACATAGCGCCTCATTTTGTGGGTCACCATATT9260.17642934442971026No Hit
GCATAGCCTGAGGATGAGTGGTTCTCAGAGGTGGAGACAGCGGGGTAGGC9000.17147560473729936No Hit
GCTTTAGGGCATGGACCTTGACCCTTATAAAGAATTTGGAGCTTCTGTGG8970.17090401938817504No Hit
TCCCGATACAGAGCCGCGGCGGTGTCTAGAAGATCTCGTACGGAAGGAAA8720.16614080814547227No Hit
GAGACGGGTCGTCCGCAGGATTCAGCGCCGACGGGACGTAAACAAAGGAC8580.16347340984955874No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT8510.16213971070160196No Hit
CCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC8470.1613775969027695No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC8390.15985336930510463No Hit
GTATCGGGATGCATTAGAGTCTCCTGAGCATGTTTCAGCTCATCATACTG8330.15871019860685598No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8320.15851967015714788No Hit
ATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACCTG8280.15775755635831543No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC8100.15432804426356944No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT8050.1533754020150289No Hit
CTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCT7800.14861219077232612No Hit
CTCTTATATAAAACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT7690.1465163778255369No Hit
AGATTGAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCG7400.1409910527840017No Hit
ATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCGCTAAATTTCATG7270.13851418293779627No Hit
CTATTTACACACTCTATGGAAGGCGGGTGTTTTATATAAGAGAGTATCAA7270.13851418293779627No Hit
GAGAAGGGTCGTCCGCAGGATTCAGCGCCGACGGGACGTAAACAAAGGAC7270.13851418293779627No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7220.13756154068925572No Hit
GGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTC7160.13641836999100707No Hit
GGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA7130.13584678464188274No Hit
CTCTAGAACCGTTTCTCTTCCAAAAGTGAGACAAGAAACGTGAAACCACA7120.1356562561921746No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT7120.1356562561921746No Hit
TCTCCATCTCGGGGATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGG6940.13222674409742863No Hit
GGTTCAGGTACTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTG6890.13127410184888808No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6830.1301309311506394No Hit
CCTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATAATACAAACCTTGCCAG6790.12936881735180697No Hit
CCCCAGAAGGCCTCCGTGCGGTGGGGTGAACCCTAGCCCGAATGCTCCAG6680.12727300440501774No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6680.12727300440501774No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6550.12479613455881232No Hit
GGGAAACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTC6520.12422454920968798No Hit
GTTAATAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTCT6500.12384349231027175No Hit
ATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCA6400.12193820781319067No Hit
CTATAAGGTGGGGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC6360.12117609401435822No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACCTTGACCCTTAT6260.11927080951727712No Hit
ATGTTTCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACCTG6250.11908028106756902No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6190.11793711036932036No Hit
GTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGG6060.11546024052311492No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6010.11450759827457437No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT5990.11412654137515814No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCCCCACC5980.11393601292545003No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG5980.11393601292545003No Hit
CTCCAAAAGACACCAAATACTCTAGAACCGTTTCTCTTCCAAAAGTGAGA5890.11222125687807703No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5860.11164967152895271No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5820.11088755773012025No Hit
CTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTTTTGCCTTCT5820.11088755773012025No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC5700.10860121633362295No Hit
GTGTCTGATGTGATCTTGTGGCAATGATCCCCAACATCCAATGACATAGC5690.10841068788391482No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACA5640.10745804563537427No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5610.10688646028624994No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5490.10460011888975262No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5490.10460011888975262No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTGTCAGTTCCGATAATGTTT5470.1042190619903364No Hit
GTTTAATACCCTTATCCAAGGGCAAATATTTGGTAAAATTAGGATAAAAC5460.10402853354062827No Hit
ACCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGAGACTT5450.10383800509092017No Hit
CTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGAC5400.10288536284237962No Hit
CTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATAATACAAACCTTGCCAGC5380.10250430594296339No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5330.10155166369442284No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5310.10117060679500664No Hit
CCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGAGACTTG5310.10117060679500664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)