FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348646
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14960.4290885310601585No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT13740.39409601716354126No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12530.3593903271513225No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA11290.32382416548590837No Hit
GCTACTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG10270.29456812927726117No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC10230.29342083373966715No Hit
ATTTTGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGATA10030.2876843560516971No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8700.24953677942669644No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA8650.24810266000470393No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8590.24638171669831288No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8560.24552124504511738No Hit
GACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTATA8010.22974593140319982No Hit
CACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTGAG7910.22687769255921478No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7910.22687769255921478No Hit
AGCTACTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAA7890.22630404479041777No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7840.22486992536842526No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA7700.22085439098684626No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG7660.21970709544925227No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT7660.21970709544925227No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7640.21913344768045526No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7620.21855979991165825No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7420.21282332222368824No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT7080.20307131015413915No Hit
CACAAATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTGAG6950.19934259965695864No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6890.19762165635056764No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACGGAGT6840.19618753692857513No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6800.1950402413909811No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6790.19475341750658262No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6520.18700917262782307No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6460.18528822932143205No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6400.18356728601504105No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6280.18012539940225902No Hit
GTGGCCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6210.1781176322114695No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6170.17697033667387552No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6120.175536217251883No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6020.17266797840789797No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6010.17238115452349947No Hit
ATTTGGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGATA5880.16865244402631896No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCA5870.16836562014192047No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5850.16779197237312346No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA5850.16779197237312346No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5620.16119502303195793No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5610.1609081991475594No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5590.1603345513787624No Hit
GTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTT5360.15373760203759687No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5340.15316395426879986No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5300.15201665873120587No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5270.15115618707801037No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5270.15115618707801037No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5200.14914841988722086No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5140.14742747658082983No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5120.14685382881203282No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5090.14599335715883732No Hit
TAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG4920.14111735112406282No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4840.13882276004887478No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG4820.13824911228007777No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4800.13767546451128077No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4780.13710181674248378No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4720.13538087343609279No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4700.13480722566729578No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4670.13394675401410028No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4540.13021804351691973No Hit
AGCTTTTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAA4520.12964439574812273No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4440.12734980467293472No Hit
GCTTTTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG4420.1267761569041377No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4320.12390791806015271No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4310.12362109417575422No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4160.11931873590977668No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4150.11903191202537818No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4140.11874508814097967No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4080.11702414483458867No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT3980.11415590599060364No Hit
ATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3950.11329543433740814No Hit
GATTCGTCCCCATGCCTTGTCGAGGTTTGGAAGACCAACCTCCCATGCTG3930.11272178656861113No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT3920.11243496268421263No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3910.11214813879981414No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3900.11186131491541564No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGT3830.10985354772462613No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC3820.10956672384022763No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT3790.10870625218703213No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG3760.10784578053383662No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3730.10698530888064112No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA3700.10612483722744559No Hit
CTCAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCAC3700.10612483722744559No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3660.10497754168985159No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3650.10469071780545308No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAACCTCTATG3640.10440389392105459No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3630.10411707003665609No Hit
CAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG3620.10383024615225758No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3610.10354342226785909No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAA3600.10325659838346059No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3580.10268295061466358No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG3540.10153565507706958No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT3520.10096200730827258No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3520.10096200730827258No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG3520.10096200730827258No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC3520.10096200730827258No Hit
CCCGCGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC3500.10038835953947557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)