FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538188
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66331.2324689513701532No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGG15600.28986153537425585No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT14270.2651489813968353No Hit
GTGTAAAGGGAGCGGCAAAACCTAAAAGACCCACAATTCTTTGACATACT14230.2644057466907475No Hit
CTCTTACAGCTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG13530.2513991393342103No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT12510.23244665432897055No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT12010.2231562205028726No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGCTAACG10660.19807204917240817No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAACGTAAGA10640.19770043181936425No Hit
CTATCTGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT10370.19268359755327133No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC10280.19101131946457373No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG9440.17540339063672916No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9260.17205883445933393No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCACAT9050.16815685225237276No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC9000.167227808869763No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8740.16239678328019205No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8700.16165354857410422No Hit
ATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT8690.16146773989758226No Hit
ATGTCAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACCTTTCC8410.1562650969549674No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7900.1467888544523475No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATCTGTTCAGG7670.14251525489234246No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT7670.14251525489234246No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7560.1404713594506009No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7440.1382416553323374No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAGC7330.13619775989059585No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7320.1360119512140739No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7260.13489709915494213No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACCTTTCC7240.13452548180189822No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6600.12263372650449286No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6460.12003240503318544No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6400.11891755297405368No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6320.11743108356187801No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGCTAACGAAAAAA6190.11501557076709255No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6110.11352910135491685No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCACATATTCATTTGAAAG5940.11037035385404358No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5850.10869807576534594No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5820.10814064973578005No Hit
CTCAAGAACAGTTTCTCTTCCAAACGTAAGACAGGAAAGGTGAAACCACA5800.10776903238273615No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCCGGGTCTTCC5740.1066541803236044No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGCTAACGAAAAAAGGA5660.10516771091142872No Hit
CTATTGATAATATCCTCTTTCAAATGAATATGTGGAAAAGAGGGAGTTTG5550.10312381546968717No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5510.10238058076359932No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5470.1016373460575115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)