FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445793
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37310.8369355283730342No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG23240.5213181902811395No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG15450.3465734096318246No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT14430.3236928350153547No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT12690.28466126655196466No Hit
CAATTCGTTGACACACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTC12440.27905328257733975No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG11490.25774294347376475No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT11460.25706998539680975No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10970.24607833680654473No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA9290.2083926844970648No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9290.2083926844970648No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8390.18820394218841482No Hit
ATATAAGACCTTGGGCAATGCCTGGCGGGCGTTCACGGTGGTCTCCATGC8250.1850634711626248No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC8040.1803527646239398No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGA7830.17564205808525482No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCATTACAAAGATC7580.17003407411062982No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7520.16868815795671982No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG7520.16868815795671982No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7500.16823951923874983No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC7480.16779088052077984No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7400.16599632564889982No Hit
CTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7370.16532336757194482No Hit
GAATTAATAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA7270.16308017398209482No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATG7130.15993970295630483No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA7060.15836946744340982No Hit
ATCCCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGA6960.15612627385355984No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6790.15231284475081486No Hit
ATAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6730.15096692859690486No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6690.15006965116096485No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA6630.14872373500705485No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGTACCTGTC6600.14805077693009983No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC6510.14603190269923486No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC6360.14266711231445986No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6300.14132119616054986No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6250.14019959936562484No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6210.13930232192968484No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG6210.13930232192968484No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA6050.13571321218592486No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6040.13548889282693985No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG5990.13436729603201486No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC5990.13436729603201486No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGA5780.12965658949332987No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACC5570.12494588295464486No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG5540.12427292487768987No Hit
CTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTG5510.12359996680073486No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTGTCTCTTCCAAAAGTAAGA5440.12202973128783987No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT5370.12045949577494487No Hit
ATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC5320.11933789898001988No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5180.11619742795422987No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5050.11328127628742488No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5040.11305695692843988No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5000.11215967949249987No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC5000.11215967949249987No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT4980.11171104077452988No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA4910.11014080526163489No Hit
GTATACATGCATACAAAGGCATCAATGCTGGGTAGCCACATTGTGTGAAG4810.10789761167178488No Hit
GTATCCATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAAC4780.10722465359482988No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT4760.10677601487685988No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC4660.10453282128700989No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATA4600.10318690513309989No Hit
GATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGT4590.10296258577411489No Hit
TTCTATTCCAAACCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCGG4570.1025139470561449No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC4560.10228962769715991No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG4520.1013923502612199No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4510.1011680309022349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)