FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445793
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37140.833122099270289No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG25710.5767250719504344No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG14890.33401152552866464No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG13080.29340972155237965No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT12940.2902692505265897No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT12290.2756884921925647No Hit
CAATTCGTTGACACACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTC11830.2653698016792547No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT10530.23620828501120472No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9940.22297344283108977No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9080.20368197795837978No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA8280.1857364292395798No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC8250.1850634711626248No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8120.1821473194958198No Hit
GAATTAATAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA8020.1799041259059698No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG7920.17766093231611982No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA7820.1754177387262698No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCATTACAAAGATC7790.1747447806493148No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGTACCTGTC7740.17362318385438982No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7730.17339886449540481No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7660.17182862898250983No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGA7600.17048271282859984No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7520.16868815795671982No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7470.1675665611617948No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC7420.16644496436686984No Hit
CTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7390.16577200628991481No Hit
ATCCCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGA7010.15724787064848483No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATG6980.15657491257152983No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6960.15612627385355984No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA6870.15410739962269485No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC6820.15298580282776983No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6810.15276148346878485No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG6340.14221847359648987No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA6310.14154551551953484No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6240.13997528000663986No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC6140.13773208641678986No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG5890.13212410244216485No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTGTCTCTTCCAAAAGTAAGA5790.12988090885231485No Hit
ATAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5760.12920795077535988No Hit
CTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTG5730.12853499269840488No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGA5700.12786203462144988No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT5600.12561884103159987No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5530.12404860551870488No Hit
ATATAAGACCTTGGGCAATGCCTGGCGGGCGTTCACGGTGGTCTCCATGC5530.12404860551870488No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG5460.12247837000580987No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5430.12180541192885487No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5380.12068381513392987No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5230.11731902474915487No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT5160.11574878923625988No Hit
ATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC5120.11485151180031987No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACC5100.11440287308234988No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT4980.11171104077452988No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA4970.11148672141554489No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT4950.11103808269757487No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4900.1099164859026499No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG4870.10924352782569488No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4840.1085705697487399No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC4800.10767329231279989No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC4740.10632737615888989No Hit
CTCACCAACCTCTTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC4730.1061030567999049No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC4700.1054300987229499No Hit
GTATACATGCATACAAAGGCATCAATGCTGGGTAGCCACATTGTGTGAAG4680.10498146000497989No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC4600.10318690513309989No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4590.10296258577411489No Hit
GTATCCATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAAC4570.1025139470561449No Hit
CTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT4470.1002707534662949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)