FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773793
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69920.9036008338147282No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG34600.44714800986827224No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT32190.4160027294121296No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT26780.3460873902968882No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA18890.24412213602345847No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC17730.22913104667527362No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG17370.22447863963618178No Hit
CTCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG16710.21594922673118006No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC16310.21077988557663355No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC16280.21039218499004256No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14820.19152408977594781No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA14670.1895855868429929No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA14670.1895855868429929No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT14510.1875178503811743No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT14460.18687168273685598No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14240.1840285451018554No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT14000.18092694040912752No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13250.17123442574435283No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13010.16813282105162491No Hit
AGTTAAGAGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA12180.15740643815594094No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCTCTTAACTTCAT11670.15081552818389415No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC11550.1492647258375302No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11480.14836009113548457No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT11450.14797239054889355No Hit
GGATATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG11370.14693852231798427No Hit
CATTTACAGGAGGATATTATTGATAGATGTCAACAATATGTGGGCCCTCT11020.14241534880775608No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA10750.13892604352843718No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT10750.13892604352843718No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA10710.13840910941298254No Hit
CCTTTAAATGAATGTTAGGGAAGGAGGGAGTTTGCCACTCAGGATTAAAG10540.1362121394223003No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC10440.13491980413366367No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA10440.13491980413366367No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9390.1213502836029791No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA9120.1178609783236602No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA9090.11747327773706921No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA9070.11721481067934189No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8880.1147593736309323No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8820.11398397245775034No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATATCCTCCTTT8780.11346703834229567No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8760.11320857128456835No Hit
ATTTAAAGGAGGATATTATTGATAGATGTCAACAATATGTGGGCCCTCTT8670.1120454695247954No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8640.1116577689382044No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8560.11062390070729511No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGT8550.11049466717843144No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8540.11036543364956779No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT8490.10971926600524948No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8330.10765152954343087No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTCC8300.10726382895683989No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATACG8250.10661766131252155No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG8180.10571302661047592No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8170.10558379308161228No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8130.10506685896615761No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAAT8120.10493762543729394No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTT8100.10467915837956664No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG8030.10377452367752099No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7940.10261142191774802No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7910.10222372133115705No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGAGAG7750.10015598486933844No Hit
ATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTAAAG7750.10015598486933844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)