FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773793
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69610.8995945944199547No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG36900.4768717215069146No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT30590.3953253647939436No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT26690.3449242885371152No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC18380.2375312260514117No Hit
CTCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG18350.2371435254648207No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC18130.23430038782982013No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA17800.23003568137731925No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC16280.21039218499004256No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16190.20922908323026962No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15980.20651517912413267No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT15810.20431820913345045No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT14770.19087792213162952No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14500.18738861685231062No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT14380.18583781450594666No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA14170.18312391039980977No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA14050.18157310805344581No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12810.16554815047435167No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12440.16076650990639615No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC12410.16037880931980517No Hit
AGTTAAGAGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA11800.15249556405912176No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT11790.15236633053025808No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA11400.14732622290457525No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCTCTTAACTTCAT11270.14564618702934765No Hit
GGATATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG10850.1402183788170738No Hit
CCTTTAAATGAATGTTAGGGAAGGAGGGAGTTTGCCACTCAGGATTAAAG10450.13504903766252732No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA10380.1341444029604817No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA10150.13117203179661743No Hit
CATTTACAGGAGGATATTATTGATAGATGTCAACAATATGTGGGCCCTCT9700.12535652299775263No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT9570.12367648712252502No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9090.11747327773706921No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC9050.11695634362161457No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8940.11553477480411428No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8840.11424243951547765No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA8830.11411320598661398No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA8780.11346703834229567No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8740.11295010422684103No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA8700.11243317011138637No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8550.11049466717843144No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT8550.11049466717843144No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8520.11010696659184045No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8440.10907309836093114No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTCC8410.10868539777434016No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8370.10816846365888551No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8350.1079099966011582No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8320.1075222960145672No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG8210.10610072719706692No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8070.10429145779297563No Hit
ATTTCAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGA8010.10351605661979367No Hit
ATTTAAAGGAGGATATTATTGATAGATGTCAACAATATGTGGGCCCTCTT7990.10325758956206633No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7980.10312835603320267No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATATCCTCCTTT7940.10261142191774802No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAAT7910.10222372133115705No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7840.1013190866291114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)