FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552600
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG34020.6156351791530945No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT27080.4900470503076367No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT17420.31523706116539996No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT16640.3011219688744119No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA16420.29714078899746654No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG15930.28827361563517917No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATG15720.28447339847991315No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCCGCTAGG15360.2779587404994571No Hit
GGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCGGGCATAATTAATTT14110.25533840028954036No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT13590.24592833876221498No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13060.23633731451321027No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA12800.23163228374954759No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12700.22982265653275427No Hit
GGTTAGGATAGAACCTAGCGGGCATAATTAATTTTAATCTCCGTTTTTCA12280.2222222222222222No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11970.21661237785016288No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11610.21009771986970682No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTCTCTAGGGGGAGCACCCGCG11160.20195439739413681No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11070.20032573289902278No Hit
GTTGTTAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11040.1997828447339848No Hit
CATTAACAACAGTGCCAACGGCGCCTCCTCCTGCCTCCACCAATCGGCAG10970.19851610568222947No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10630.1923633731451321No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10510.1901918204849801No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA10420.1885631559898661No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAACTTGGTGGAATGT10370.18765834238146942No Hit
ATACTTCCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA10220.1849439015562794No Hit
CTCCCACCTGATTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC10220.1849439015562794No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT10220.1849439015562794No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG10070.1822294607310894No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC10050.18186753528773072No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9740.17625769091567137No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9460.17119073470865No Hit
GTAATCTGGAAGACCCAACATCCAGGGAAGCAGTAGTCAGCTATGTCAAT9090.16449511400651465No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA9050.1637712631197973No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8940.16178067318132466No Hit
GGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATG8790.15906623235613465No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8760.15852334419109662No Hit
CTAACAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA8670.15689467969598264No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8500.15381831342743396No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8410.15218964893231993No Hit
ATACTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG8150.14748461816865727No Hit
TCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAACTCACCCCAACA8140.1473036554469779No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8100.1465798045602606No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8100.1465798045602606No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8020.14513210278682592No Hit
ATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG7960.14404632645674992No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7910.14314151284835322No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7870.14241766196163588No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7840.1418747737965979No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7760.14042707202316324No Hit
CCTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC7650.13843648208469056No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7640.1382555193630112No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7620.13789359391965253No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7400.1339124140427072No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7310.1322837495475932No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7270.13155989866087586No Hit
GGTCCGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCC7250.1311979732175172No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7220.1306550850524792No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7170.12975027144408252No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT7160.1295693087224032No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7100.12848353239232718No Hit
GGATAGAACCTAGCGGGCATAATTAATTTTAATCTCCGTTTTTCATTAAC7100.12848353239232718No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTCATTACAATA6980.12631197973217517No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6690.12106406080347448No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6680.12088309808179515No Hit
ATGTGGGCCCTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATG6670.12070213536011581No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6660.12052117263843648No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6600.11943539630836049No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC6520.11798769453492582No Hit
CATTTGAACCCTAACAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6470.11708288092652913No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6440.11653999276149113No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6330.11454940282301847No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6260.11328266377126311No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6210.11237785016286646No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT6180.11183496199782844No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6110.1105682229460731No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6070.10984437205935577No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCT6040.10930148389431778No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5980.10821570756424177No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT5920.10712993123416577No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5880.10640608034744843No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5870.10622511762576908No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG5850.10586319218241043No Hit
ATCACCAGTTGGACCCAGCGTTCGGAGCCAACTCAAACAATCCAGATTGG5830.10550126673905176No Hit
GCTAGGAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAAGTTCCACG5790.10477741585233442No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5760.10423452768729641No Hit
ACTGATGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTC5750.10405356496561709No Hit
GTATGGATCGGCAGAGGAGCCACAAAAGTTCCACGCATGCGCTGATGGCC5710.10332971407889975No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT5690.10296778863554108No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5680.10278682591386173No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5630.10188201230546508No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5610.10152008686210641No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTCATTACAAT5580.10097719869706841No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5580.10097719869706841No Hit
CTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG5550.10043431053203042No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5530.10007238508867175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)