FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547647
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTAAAGTAATGATTAACTACATGTTCAGG19370.35369498965574536No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18290.33397425714009205No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13670.2496133458231306No Hit
GTGTAAAAGGTGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13460.2457787589450869No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12840.23445759768610072No Hit
CCTATATAGAATGCCAGCCTTCCACAAAGTATGTAAATAATGCCTGGTTT11750.21455426579530243No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11570.21126747704269355No Hit
ATAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA11410.20834588704037454No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC11200.20451130016233085No Hit
CTCCTATATAGAATGCCAGCCTTCCACAAAGTATGTAAATAATGCCTGGT10490.19154674452704024No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10400.18990335015073578No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10090.1842427695212427No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10080.18406017014609774No Hit
ATAACCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA10060.1836949713958079No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9910.1809559807686338No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9620.17566059888943059No Hit
CCTCCCCCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9600.17529540013914074No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG9360.1709130151356622No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9310.17000001825993752No Hit
CTATAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC9150.16707842825761848No Hit
GTTTTAAAGTAATGATTAACTACATGTTCAGGATAATAAGGTTTAATGCC9110.16634803075703875No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8920.16287864262928492No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8630.15758326075008172No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8560.15630506512406714No Hit
GGTCAGGACTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGC8540.15593986637377727No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8520.15557466762348737No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8300.15155748137029876No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT8170.14918368949341454No Hit
ATATAGGAGAGAAACTACACACAGCGCCTCATTTTGTGGGTCACCATATT8000.1460795001159506No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7950.1451665032402259No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA7750.14151451573732715No Hit
CTATATAGGAGAGAAACTACACACAGCGCCTCATTTTGTGGGTCACCATA7750.14151451573732715No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGACTATGCCAAGTGTTTGCTG7720.14096671761189233No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7560.13804512760957333No Hit
GTCCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGTTTACAC7410.13530613698239924No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7310.13348014323094987No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7220.1318367488546454No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC7210.13165414947950047No Hit
GGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA7150.13055855322863086No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA7130.13019335447834096No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCAAG7050.12873255947718146No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAA6940.12672396635058714No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6830.12471537322399281No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6830.12471537322399281No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAGCA6820.1245327738488479No Hit
CAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCATGGGATATGTAA6750.12325457822283331No Hit
TCCTCAGGCCGTGCAGTGGAACTCCACAACATTCCACCAAGCTCTGATAG6580.12015038884536937No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTAAGTACAATA6560.11978519009507949No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6460.1179591963436301No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6210.11339421196500665No Hit
CCCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA6190.11302901321471677No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT6150.11229861571413703No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6150.11229861571413703No Hit
ATATTGTACTTAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA6120.11175081758870221No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGAC6030.11010742321239776No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5970.10901182696152814No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5950.10864662821123826No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5840.10663803508464394No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5770.10535983945862938No Hit
GTGTGTAGTTTCTCTCCTATATAGAATGCCAGCCTTCCACAAAGTATGTA5730.10462944195804962No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5730.10462944195804962No Hit
CAATCAAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTG5720.10444684258290467No Hit
GTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCA5640.10298604758174516No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5580.10189045133087554No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5530.10097745445515084No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5530.10097745445515084No Hit
TTATTATCCTGAACATGTAGTTAATCATTACTTTAAAACCAGGCATTATT5520.10079485508000592No Hit
CATGTATACAATCAAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5480.10006445757942617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)