FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547647
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTAAAGTAATGATTAACTACATGTTCAGG20360.3717723277950943No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18220.3326960615140775No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13670.2496133458231306No Hit
GTGTAAAAGGTGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13650.2492481470728407No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13600.24833515019711602No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12530.22879701705660763No Hit
CTCCTATATAGAATGCCAGCCTTCCACAAAGTATGTAAATAATGCCTGGT11690.21345866954443285No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC11320.2067024926640701No Hit
CCTATATAGAATGCCAGCCTTCCACAAAGTATGTAAATAATGCCTGGTTT11240.2052416976629106No Hit
CCTCCCCCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10990.20067671328428713No Hit
GTTTTAAAGTAATGATTAACTACATGTTCAGGATAATAAGGTTTAATGCC10860.19830292140740297No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10500.19172934390218516No Hit
ATAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA10450.19081634702646047No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10160.18552096514725727No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9920.18113858014377873No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9690.17693879451544517No Hit
ATAACCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA9310.17000001825993752No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9290.16963481950964765No Hit
CTATAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC9180.1676262263830533No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9160.16726102763276343No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG9140.16689582888247356No Hit
GGTCAGGACTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGC8740.15959185387667604No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTAAGTACAATA8640.15776586012522664No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8280.1511922826200089No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8250.15064448449457404No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8220.15009668636913925No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8080.14754029511711012No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7990.14589690074080566No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCAAG7880.14388830761421134No Hit
ATATAGGAGAGAAACTACACACAGCGCCTCATTTTGTGGGTCACCATATT7810.14261011198819679No Hit
CTATATAGGAGAGAAACTACACACAGCGCCTCATTTTGTGGGTCACCATA7770.14187971448761702No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7700.14060151886160246No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7670.14005372073616762No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGACTATGCCAAGTGTTTGCTG7460.13621913385812395No Hit
GGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA7290.13311494448066No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT7250.13238454698008023No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC7220.1318367488546454No Hit
GTCCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGTTTACAC7030.1283673607268916No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7020.12818476135174664No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA6970.12727176447602195No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6960.12708916510087703No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6780.12380237634826814No Hit
ATATTGTACTTAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA6770.1236197769731232No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6510.1188721932193548No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6410.11704619946790543No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT6400.11686360009276048No Hit
TCCTCAGGCCGTGCAGTGGAACTCCACAACATTCCACCAAGCTCTGATAG6390.11668100071761554No Hit
GTGTGTAGTTTCTCTCCTATATAGAATGCCAGCCTTCCACAAAGTATGTA6390.11668100071761554No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6360.11613320259218074No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAA6270.11448980821587627No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAGCA6270.11448980821587627No Hit
CAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCATGGGATATGTAA6170.11266381446442691No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC6100.11138561883841233No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6090.11120301946326738No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5970.10901182696152814No Hit
GGTTGGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5910.1079162307106585No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGAC5880.1073684325852237No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5820.10627283633435405No Hit
CCCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5770.10535983945862938No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5720.10444684258290467No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5710.10426424320775976No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTTAAA5630.10280344820660023No Hit
CTCTTATATAGAATGCCAGCCTTCCACAAAGTATGTAAATAATGCCTGGT5630.10280344820660023No Hit
TTATTATCCTGAACATGTAGTTAATCATTACTTTAAAACCAGGCATTATT5620.1026208488314553No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTAAGTACAAT5590.10207305070602049No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5570.10170785195573061No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5560.10152525258058567No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5550.10134265320544072No Hit
TTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCG5480.10006445757942617No Hit
ACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTT5480.10006445757942617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)