FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517989
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTAAAATAATGATTAACTGCATGTTCAGG20270.39132105121923433No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA19590.3781933593184411No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG17550.33881028361606136No Hit
CTTATATAGGATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT15720.30348134805951477No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG15220.29382863342657856No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12520.24170397440872296No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12360.23861510572618339No Hit
GGTTAGGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA11380.21969578504562837No Hit
ATATGTTCCTGTGGTAAAGTTCCCCAACTTCCAATTACATATCCCATAAA10150.1959501070486053No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGAACTTTACCACAG10060.19421261841467677No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC9540.1841737951964231No Hit
GGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC9280.17915438358729624No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9140.1764516234900741No Hit
CTCTTATATAGGATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTGGT8990.17355580910019325No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8770.1693086146617013No Hit
TATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAAA8530.16467531163789192No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8460.16332393158928085No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8020.15482954271229696No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7780.1501962396884876No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC7740.1494240225178527No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGT7610.14691431671328928No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7610.14691431671328928No Hit
CTCCAAAAGACACCAGATACTCAAGAACAGTTTGTCTTCCAAAAGTAAGA7510.14498377378670205No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7420.14324628515277352No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7400.14286017656745606No Hit
GTATACATGCGTATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7370.1422810136894799No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7350.14189490510416244No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7300.14092963364086883No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7060.13629633061705942No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7060.13629633061705942No Hit
GGGTATACATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTA7030.13571716773908327No Hit
TTTTAGTACAATATGTTCCTGTGGTAAAGTTCCCCAACTTCCAATTACAT6940.13397967910515474No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6680.1289602674960279No Hit
CAAGCAGATGAGGCATAGCAGCAGGATGAAGAGGAATATGATAAAACGCC6660.12857415891071047No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6500.12548529022817087No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6490.12529223593551214No Hit
GAGTATGTAAATAATGCCTGGTTTTAAAATAATGATTAACTGCATGTTCA6350.12258947583829001No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6320.12201031296031382No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTAAAATAATGATTAACTGCA6320.12201031296031382No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGGTT6280.12123809578967894No Hit
TCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTG6250.12065893291170275No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG6230.1202728243263853No Hit
CTCAAGAACAGTTTGTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6230.1202728243263853No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTCA5990.11563952130257593No Hit
TCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5940.1146742498392823No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5800.11197148974206016No Hit
GCTTTAGGGCATGGACATTGACGTATATAAAGAATTTGGAGCCTCTGTGG5790.11177843544940144No Hit
TTGACATACTTTCCAATCAATAGGTCTATTGACAGGCAGTTTCCGAAAAC5790.11177843544940144No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATACGCATGTATA5760.11119927257142526No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT5760.11119927257142526No Hit
GTTTTAAAATAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5740.11081316398610783No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC5680.10965483823015547No Hit
CCTTTATACGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAAC5660.10926872964483801No Hit
TCATCATACAGCACTCAGGCAAGCCATTCTATGTTGGGGTGAGTTAATGA5620.10849651247420311No Hit
ATTTACATACTCTGTGGAAGGCGGGCATCCTATATAAGAGAGAAACTACA5590.10791734959622694No Hit
CCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG5590.10791734959622694No Hit
ATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAG5550.10714513242559205No Hit
TTATTATCCTGAACATGCAGTTAATCATTATTTTAAAACCAGGCATTATT5430.10482848091368736No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5430.10482848091368736No Hit
CATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT5420.10463542662102862No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5390.10405626374305246No Hit
ACACCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGG5370.103670155157735No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5350.10328404657241755No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC5310.10251182940178266No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5300.10231877510912393No Hit
TTTACATACTCTGTGGAAGGCGGGCATCCTATATAAGAGAGAAACTACAC5290.10212572081646522No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG5210.1005812864751954No Hit
GCCTTAAAGGTTCCACGCATGCGCCGATGGCCTACGGCCAAGCCCCATCC5190.10019517788987797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)