FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517989
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTAAAATAATGATTAACTGCATGTTCAGG23040.444797090285701No Hit
CTTATATAGGATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT17570.33919639220137876No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA16940.32703397176387916No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG14780.2853342445495947No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG14160.27336487840475376No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12320.2378428885555485No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9970.19247512978074824No Hit
CTCTTATATAGGATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTGGT9870.19054458685416098No Hit
GGTTAGGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA9760.18842098963491502No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGT9070.17510024344146302No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8750.16892250607638384No Hit
ATATGTTCCTGTGGTAAAGTTCCCCAACTTCCAATTACATATCCCATAAA8700.16795723461309026No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8540.16486836593055065No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8280.15984895432142382No Hit
GGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC8220.15869062856547148No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGAACTTTACCACAG8050.15540870559027314No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8000.15444343412697953No Hit
CTCCAAAAGACACCAGATACTCAAGAACAGTTTGTCTTCCAAAAGTAAGA7710.14884485963987654No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC7470.14421155661606713No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7080.13668243920237688No Hit
GTATACATGCGTATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7040.135910222031742No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6990.13494495056844835No Hit
TATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAAA6820.13166302759325005No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6650.12838110461805172No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6560.12664361598412321No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6380.12316863871626618No Hit
GAGTATGTAAATAATGCCTGGTTTTAAAATAATGATTAACTGCATGTTCA6220.12007977003372658No Hit
GGGTATACATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTA6220.12007977003372658No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6070.11718395564384572No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5960.11506035842459976No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5900.1139020326686474No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5740.11081316398610783No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5620.10849651247420311No Hit
TTTTAGTACAATATGTTCCTGTGGTAAAGTTCCCCAACTTCCAATTACAT5570.10753124101090951No Hit
CCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG5550.10714513242559205No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG5530.10675902384027461No Hit
CTCAAGAACAGTTTGTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5520.10656596954761587No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5490.1059868066696397No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATACGCATGTATA5480.10579375237698098No Hit
GTTTTAAAATAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5440.10502153520634608No Hit
CCTTTATACGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAAC5390.10405626374305246No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5390.10405626374305246No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5290.10212572081646522No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC5270.10173961223114776No Hit
GCTTTAGGGCATGGACATTGACGTATATAAAGAATTTGGAGCCTCTGTGG5220.10077434076785415No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5190.10019517788987797No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5180.10000212359721926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)