FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521373
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24180.46377545442514284No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT22900.4392248927351436No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA21550.4133317222027224No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT19290.36998463671881743No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCAGG19270.3696010341924112No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA16890.3239523335500688No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG15860.3041968034401475No Hit
GTTAATATCCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTC15260.29268872764796033No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14840.2846330745934293No Hit
CTATCAATAATGTCCTCCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTG14760.2830986644878043No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12800.24550561689999292No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT10900.2090633768914002No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10500.20139132636327542No Hit
CTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT10160.19487008341436937No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9730.18662262909663524No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC9660.18528002025421342No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9540.18297840509577595No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATG9490.18201939877976037No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9450.1812521937269479No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8560.16418188130187025No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8500.16303107372265152No Hit
CTATTAATAATGTCCTCCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTG8480.16264747119624529No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAAA8470.16245566993304217No Hit
GCATATTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTT8380.1607294585642141No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8270.1586196446689798No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8190.15708523456335485No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7840.15037219035124563No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7700.147686972666402No Hit
GAACATACAGTTAATCATTACTTCAAAACTAGGCATTATTTACATACTCT7690.14749517140319887No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7350.1409739284542928No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7350.1409739284542928No Hit
ACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATA7340.14078212719108968No Hit
ATTGATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAAAG7230.1386723132958554No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7200.138096909506246No Hit
ATTTAAAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTT7190.1379051082430429No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTAAAGG6960.13349367918937113No Hit
GGGAAGCAGTAGTCAGCTATGTCAATGTTAATATGGGCCTGAAATTCAGA6840.1311920640309337No Hit
CTGCATATTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT6780.130041256451715No Hit
ATGTTAATATGGGCCTGAAATTCAGACAACTATTGTGGTTTCATATTTCC6720.1288904488724963No Hit
TATTGATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAAA6660.12773964129327756No Hit
GGATATTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6600.12658883371405885No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6510.12486262234523077No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCA6500.12467082108202764No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6440.12352001350280893No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6360.12198560339718398No Hit
CATTTAAAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT6320.12121839834437151No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAAT6010.1152725591850748No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6000.11508075792187168No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5930.11373814907944983No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5930.11373814907944983No Hit
CCTCAAGCTTACAGACCACCCAATGCCCCTATCTTATCAACACTTCCGGA5880.11277914276343423No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATT5860.112395540237028No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5840.11201193771062178No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT5840.11201193771062178No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAATATGTT5790.11105293139460617No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5750.11028572634179368No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAATA5750.11028572634179368No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5740.11009392507859057No Hit
CAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATATGAAACCACAAT5730.10990212381538744No Hit
CATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG5710.10951852128898122No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5670.10875131623616872No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA5520.10587429728812195No Hit
GTTTTGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCC5520.10587429728812195No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5450.1045316884457001No Hit
CTCCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAG5340.1024218745504658No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAGATC5330.10223007328726266No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTAATGAAA5330.10223007328726266No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5280.10127106697124708No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG5270.10107926570804394No Hit
ATTAACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5240.10050386191843459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)