FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521373
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24140.4630082493723303No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCAGG21860.4192775613620191No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT20670.3964532110408479No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA20310.3895483655655356No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18720.3590519647162396No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA16230.3112934501786629No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA16160.309950841336241No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16000.30688202112499113No Hit
CTATCAATAATGTCCTCCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTG14710.2821396581717887No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT14580.2796462417501482No Hit
GTTAATATCCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTC14130.2710151849060078No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT11600.22248946531561856No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10960.21021418447061893No Hit
CTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT10660.20446014657452533No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10130.19429467962476No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC9980.19141766067671323No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9760.1871980328862446No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9610.1843210139381978No Hit
CTATTAATAATGTCCTCCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTG9160.17568995709405744No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8510.16322287498585467No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8490.16283927245944843No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATG8440.16188026614343282No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8310.15938684972179226No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAAA7910.15171479919366748No Hit
GCATATTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTT7870.150947594140855No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTAAAGG7850.15056399161444878No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7850.15056399161444878No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7490.14365914613913647No Hit
CTGCATATTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT7490.14365914613913647No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7470.14327554361273023No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7290.1398231208750741No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAATA7160.13732970445343354No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7080.13579529434780857No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6930.13291827539976178No Hit
ACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATA6890.1321510703469493No Hit
GGATATTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6830.1310002627677306No Hit
ATGTTAATATGGGCCTGAAATTCAGACAACTATTGTGGTTTCATATTTCC6720.1288904488724963No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6580.1262052311876526No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6570.12601342992444947No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6470.12409541729241828No Hit
GAACATACAGTTAATCATTACTTCAAAACTAGGCATTATTTACATACTCT6440.12352001350280893No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCA6370.12217740466038708No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAAT6360.12198560339718398No Hit
ATTGATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAAAG6320.12121839834437151No Hit
ATTTAAAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTT6300.12083479581796526No Hit
TATTGATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAAA6250.11987578950194966No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6220.1193003857123403No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6180.11853318065952782No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6050.11603976423788726No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6010.1152725591850748No Hit
CCTCAAGCTTACAGACCACCCAATGCCCCTATCTTATCAACACTTCCGGA5930.11373814907944983No Hit
CATTTAAAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT5930.11373814907944983No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTAATGAAA5930.11373814907944983No Hit
GTTTTGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCC5850.11220373897382488No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5840.11201193771062178No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATT5790.11105293139460617No Hit
CTCCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAG5780.11086113013140304No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG5750.11028572634179368No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA5730.10990212381538744No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT5710.10951852128898122No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5700.10932672002577808No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAATATGTT5660.10855951497296562No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT5520.10587429728812195No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5470.10491529097210635No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5340.1024218745504658No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG5310.10184647076085644No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5270.10107926570804394No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5230.10031206065523147No Hit
GCCTGAGGGTGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5220.10012025939202834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)