FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549705
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT15900.2892460501541736No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14950.27196405344684876No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13700.24922458409510553No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT12360.22484787295003686No Hit
GTTGTTAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11690.2126595173775025No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTTCTTTGCCACAG11350.20647438171382831No Hit
CTTATATAAAATGCCAGCCTTCCACAGCATATGTAAATAATGCCTAGTTT11140.20265415086273547No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11070.20138074057903785No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11030.20065307755978207No Hit
CATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG10840.1971966782183171No Hit
GTATACATGCATATAAAGGCTTCAAGGCAGGATAGCCACATTGTGTAAAA10620.1931945316124103No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10590.19264878434796845No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT10310.18755514321317798No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG10070.1831891650976433No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC9530.1733657143376902No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9530.1733657143376902No Hit
CTACTGTACCGGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8600.15644754913999329No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8480.15426456008222592No Hit
GTTTAGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCATCATACAG8400.15280923404371435No Hit
ATTTAGAAGACCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTT8280.150626244985947No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8000.14553260385115652No Hit
GCATATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8000.14553260385115652No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGCATATGTAAATAATGCCTAGT7950.1446230250770868No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7750.14098470998080787No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7670.1395293839422963No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7220.13134317497566877No Hit
TATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGA7120.1295240174275293No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTCATC7120.1295240174275293No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTCATCACAATA7060.12843252289864562No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7060.12843252289864562No Hit
ATATGTTCCTGTGGCAAAGAACCCCAACTTCCAATTACATATCCCATGAA6980.12697719686013406No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCCGGTTACAGACCACAAAATGCC6970.12679528110532012No Hit
AGTCTAGCCTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6780.12333888176385517No Hit
TTCTTCTACTGTACCGGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6760.12297505025422727No Hit
TCCCTAAACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATGGAAGGAAA6710.12206547148015753No Hit
GGCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG6500.11824524062906468No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6300.11460692553278576No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6290.11442500977797182No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6260.11387926251352999No Hit
CAATCGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTCATC6250.11369734675871603No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6250.11369734675871603No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6200.11278776798464632No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6140.11169627345576263No Hit
CTCTAAGGCCTCCCTAAACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6110.11115052619132079No Hit
GTAGAGGCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6100.11096861043650685No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6070.11042286317206502No Hit
GGCCTCTACCGTCCGCTTCTTCATCTGCCGTTCCGACCGACCACGGGGCG6010.10933136864318134No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6000.1091494528883674No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6000.1091494528883674No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5940.10805795835948373No Hit
CTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCTACTGTACCGGTC5810.10569305354690244No Hit
ATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTCATCACAATATGTT5780.1051473062824606No Hit
GTACAGTAGAAGAATAAAGACCAGTAAAGTTTCCCACCTTATGAGTCCAA5730.10423772750839087No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCTACTG5580.10150899118618167No Hit
TCATCATACAGCACTCAGGCAAGCTATTCTGTGCTGGGGTGAGTTGATGA5570.10132707543136774No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5550.10096324392173985No Hit
CATATAAAGGCTTCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5540.1007813281669259No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAA5530.10059941241211195No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5530.10059941241211195No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGTCTTTATTC5530.10059941241211195No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGAAGCCTTTATATGCATGTATA5510.10023558090248405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)