FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395362
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19170.48487209190564595No Hit
GTATGTAAGTAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12600.31869527167507244No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAGTAATGCCTAGTTT12320.31161315452673755No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT11340.2868257445075652No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9750.24660943641523467No Hit
CTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGGGAAACTACAC8500.21499284200302507No Hit
CTATATAAGAGGGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA8200.20740485934409475No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7980.2018403387275459No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7540.19071129749444812No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7020.17755879421896895No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6980.17654706319777824No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6780.1714884080918247No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6710.16971787880474098No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6640.16794734951765725No Hit
CCTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6390.16162403063521533No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6270.15858883757164322No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6130.15504777899747574No Hit
GCACGGGACCATGCAAAACCTGCACGATTCCTGCTCAAGGAACCTCTATG5850.14796566184914078No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT5590.1413894102114012No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5460.1381012843925314No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5460.1381012843925314No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5380.13607782235014998No Hit
GGATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5310.13430729306306624No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5260.13304262928657787No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA5190.13127209999949413No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTCCCTAATATTCG5150.13026036897830343No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAGTAATGCCTAGT5090.12874277244651736No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5080.1284898396912197No Hit
GAGTATGTAAGTAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5030.12722517591473131No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA5020.12697224315943362No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA4940.12494878111705221No Hit
GTGTGTAGTTTCCCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA4940.12494878111705221No Hit
GTATACAGGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA4880.12343118458526617No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4840.12241945356407545No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4810.12166065529818243No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4740.11989012601109868No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4660.11786666396871727No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTGTCTCCCAAAAGTGAGA4640.11736079845812192No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4620.11685493294752657No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4560.11533733641574051No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4560.11533733641574051No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4530.11457853814984748No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4510.11407267263925214No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4510.11407267263925214No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4430.11204921059687072No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4380.11078454682038233No Hit
ATATAAGAGGGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT4360.11027868130978698No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4350.11002574855448931No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4350.11002574855448931No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTTTTGCATGGTCCCGTG4320.10926695028859627No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4300.10876108477800094No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4270.10800228651210789No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAAT4180.1057258917144288No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTT4170.10547295895913113No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4140.1047141606932381No Hit
GGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAG4100.10370242967204737No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4090.1034494969167497No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4080.10319656416145204No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4080.10319656416145204No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGGGAAACTACACAC4030.10193190038496366No Hit
ACCCAGCATCCAGGGAAGCAGTAGTCAGCTATGTCAATGTTAATATGGGC4020.10167896762966597No Hit
CCTCCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3990.10092016936377295No Hit
CTGTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC3990.10092016936377295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)