FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486546
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43550.895084945719418No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA22070.45360562002359484No Hit
CTTTTTACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA14720.30254076695728666No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14580.2996633411845951No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14510.2982246282982493No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13270.2727388571686952No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12350.25383005923386487No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11780.24211482573076337No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10340.2125184463545071No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9370.1925819963580011No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGCGTAAAA8860.1820999453289103No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8320.1710013030628142No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8310.1707957726504791No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8240.16935705976413332No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8200.16853493811479284No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA8140.16730175564078217No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8070.16586304275443636No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT7630.1568197046116914No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7600.15620311337468606No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA7590.15599758296235094No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7580.15579205255001582No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7450.15312015718965935No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7260.14921507935529219No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6700.13770537626452586No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6630.13626666337818008No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6590.13544454172883963No Hit
AGACTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT6580.1352390113165045No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6500.13359476801782358No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6460.13277264636848313No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6230.12804544688477554No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTGTCAACACTTCCGGA6200.1274288556477702No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6160.12660673399842975No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6090.12516802111208397No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5960.12249612575172747No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5910.12146847369005191No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5580.11468597008299317No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5570.11448043967065807No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5570.11448043967065807No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5520.1134527876089825No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5470.11242513554730693No Hit
GATTTAGATCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT5280.10852005771293978No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGTCTTGGCCCCCAATACCACAT5260.10810899688826955No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5200.10687581441425889No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5100.10482051029090775No Hit
GTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5100.10482051029090775No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGCGTAAAAGGGGCAGCA4940.10153202369354594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)