FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486546
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43290.8897411549987052No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA20730.4260645447706897No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14470.2974025066489088No Hit
CTTTTTACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA13580.2791102999510838No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12890.26492870149996095No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12310.2530079375845244No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11890.2443756602664496No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11740.24129270408142292No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10200.2096410205818155No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8980.18456631027693168No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGCGTAAAA8750.17983911079322407No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8350.17161789429981955No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7950.163396677806415No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA7940.1631911473940799No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7910.16257455615707456No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7830.16093031285839365No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7820.16072478244605856No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT7730.1588750087350425No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7690.15805288708570206No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT7440.15291462677732426No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7370.15147591389097845No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7190.1477763664689464No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7180.1475708360566113No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6980.14346022780990902No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6740.13852749791386632No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6570.1350334809041694No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6460.13277264636848313No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6290.1292786293587862No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6270.128867568534116No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTGTCAACACTTCCGGA6130.1259901427614244No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6120.1257846123490893No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6110.12557908193675418No Hit
GGATAGCCACATTGCGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5940.12208506492705726No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5930.12187953451472215No Hit
AGACTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT5780.11879657832969545No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5540.11386384843365273No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5280.10852005771293978No Hit
GATTTAGATCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT5260.10810899688826955No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5240.10769793606359933No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5160.10605369276491843No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5100.10482051029090775No Hit
GTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5050.10379285822923218No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5000.1027652061675566No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4930.10132649328121082No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGTCTTGGCCCCCAATACCACAT4900.10070990204420548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)