FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443694
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23820.5368564821701444No Hit
CTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGTTT18490.4167286463193101No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12670.2855571632701817No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA11490.25896225777224846No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10680.2407064328118027No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGT10640.23980491059153378No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10480.2361988217104581No Hit
CTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTCCTAAC10380.23394501615978577No Hit
CCCTCATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATG9780.2204221828557519No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9150.20622320788651638No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9100.2050963051111802No Hit
CTATTAAGAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9020.20329326067064238No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8660.19517956068822206No Hit
TTCTTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8550.19270037458248251No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAAGAATGTCCTCCTGT7960.1794029218335159No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7770.1751206912872385No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7760.17489531073217127No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7680.17309226629163343No Hit
ATATGTTCCTGTGGCAAAGTTCCCCAACTTCCAATTACATATCCCATGAA7600.17128922185109557No Hit
AACTCAGACAAATATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA7360.16588008852948202No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA7340.16542932741934757No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7280.1640770440889442No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7050.15889329132239788No Hit
TTCTTCTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTC6670.15032883022984309No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6350.1431166524676917No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6260.1410882274720866No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5710.1286922969433889No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5710.1286922969433889No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5660.12756539416805274No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCA5520.12441006639711152No Hit
TATGCAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATG5490.12373392473190982No Hit
GCGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5380.1212547386261703No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5240.11809941085522906No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5200.11719788863496013No Hit
GTACAATATGTTCCTGTGGCAAAGTTCCCCAACTTCCAATTACATATCCC5180.11674712752482568No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5130.1156202247494895No Hit
CCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATG5120.1153948441944223No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5060.1140425608640189No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5000.1126902775336155No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4990.11246489697854828No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4960.11178875531334659No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4940.11133799420321212No Hit
TTATGAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA4860.10953494976267428No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4820.10863342754240536No Hit
CCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG4790.10795728587720366No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4760.10728114421200198No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG4690.10570348032653135No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4600.10367505533092627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)