FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443694
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23620.5323488710687997No Hit
CTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGTTT16270.36669416309438485No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA14060.3168850604245268No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12140.27361199385161844No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT11530.25986377999251736No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11400.2569338327766434No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGT9860.2222252272962898No Hit
CTATTAAGAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9290.20937853565745762No Hit
CCCTCATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATG8790.1981095079040961No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8770.19765874679396161No Hit
TTCTTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8460.19067194958687744No Hit
CTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTCCTAAC8220.1852628162652639No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8180.184361294044995No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8050.181431346829121No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8020.18075520516391927No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7870.17737449683791082No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7830.1764729746176419No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7600.17128922185109557No Hit
AACTCAGACAAATATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA7450.16790851352508712No Hit
ATATGTTCCTGTGGCAAAGTTCCCCAACTTCCAATTACATATCCCATGAA7310.16475318575414588No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAAGAATGTCCTCCTGT7040.15866791076733064No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6590.14852578578930525No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6400.14424355524302784No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6400.14424355524302784No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6340.14289127191262446No Hit
TTCTTCTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTC6320.14244051080249No Hit
GTACAATATGTTCCTGTGGCAAAGTTCCCCAACTTCCAATTACATATCCC5970.1345521913751369No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5960.13432681082006967No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5700.1284669163883217No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5610.1264384913927166No Hit
TTATGAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA5570.1255369691724477No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5470.12328316362177535No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5310.11967707474069968No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5230.11787403030016183No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5190.11697250807989289No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5070.11426794141908612No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4900.1104364719829432No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT4820.10863342754240536No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4780.10773190532213643No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC4520.10187201089038843No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGAACTTTGCCACAG4460.10051972755998505No Hit
CCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG4460.10051972755998505No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4460.10051972755998505No Hit
CCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATG4460.10051972755998505No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4440.10006896644985058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)