FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342123
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTTAATGAAAAAAGGAGATTAAGGTTAATTATGCCTGCTAGG15000.43843880709569366No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12680.370626938264893No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11510.33642871131142893No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG10750.3142144784185804No Hit
GGTTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCC9320.27241664547545763No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8710.2545868006535661No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8050.23529549314135562No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAACCTTAATCTCCTTTTTTCA8050.23529549314135562No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7890.23061881253233488No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT7270.21249667517237955No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7030.20548165425884843No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6600.1929130751221052No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6560.19174390496985003No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6030.17625240045246884No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATA5950.1739140601479585No Hit
CCCTTAACTTCATGGGATATGTCATTGGAAGTTGGGGTACTTTACCACAG5890.1721603049195757No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5440.15900714070670488No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5400.1578379705544497No Hit
CTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT5360.15666880040219455No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAGGTTAATTATGCCTGCTAGGTT5020.14673085410802547No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACATTGCTGAGAGTCCAA4920.1438079287273875No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA4900.14322334365125994No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATGACATATCCCATGAA4830.14117729588481337No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA4710.1376697854280478No Hit
TAATTAACCTTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATAT4690.1370852003519202No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG4670.13650061527579263No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4570.13357768989515467No Hit
GGATAAAACCTAGCAGGCATAATTAACCTTAATCTCCTTTTTTCATTAAC4540.13270081228096328No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4530.1324085197428995No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4490.13123934959064432No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT4310.12597808390549597No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4240.1239320361390494No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATA4170.12188598837260284No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG4160.12159369583453904No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAAT4140.12100911075841145No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4130.12071681822034765No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATATT4120.12042452568228387No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT4100.11983994060615626No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4070.11896306299196487No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG4040.1180861853777735No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC4020.11750160030164589No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3990.11662472268745452No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC3990.11662472268745452No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT3990.11662472268745452No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC3980.11633243014939072No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC3970.11604013761132692No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3940.11516325999713553No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG3940.11516325999713553No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3900.11399408984488035No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAGGTTAAT3840.11224033461649757No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT3840.11224033461649757No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG3810.11136345700230618No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3770.110194286850051No Hit
ATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTT3750.10960970177392342No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATATGTT3710.10844053162166822No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3700.10814823908360445No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3700.10814823908360445No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT3700.10814823908360445No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3680.10756365400747683No Hit
GGCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3660.10697906893134924No Hit
GCTTTAGGGCATGGACATCGACCCGTATAAAGAATTTGGAGCTTCTGTGG3580.10464072862683889No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3570.10434843608877507No Hit
GATTAAGGTTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT3540.10347155847458371No Hit
CCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGC3530.10317926593651991No Hit
AGTCTAGCCTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3460.10113321817007334No Hit
GAAAAAAGGAGATTAAGGTTAATTATGCCTGCTAGGTTTTATCCTAACCT3450.10084092563200954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)