FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342123
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTTAATGAAAAAAGGAGATTAAGGTTAATTATGCCTGCTAGG13900.40628662790867615No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11990.3504587531384911No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11830.3457820725294704No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG10580.3092455052714959No Hit
GGTTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCC9430.27563186339415946No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8420.24611031704971603No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8180.23909529613618496No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7840.22915734984201588No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7830.2288650573039521No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT7550.2206808662381658No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAACCTTAATCTCCTTTTTTCA7510.21951169608591062No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6710.19612829304080695No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6580.1923284900459776No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6300.18414429898019133No Hit
CTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT5890.1721603049195757No Hit
CCCTTAACTTCATGGGATATGTCATTGGAAGTTGGGGTACTTTACCACAG5880.17186801238151192No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5820.17011425715312914No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5600.16368382131572562No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATA5560.16251465116347044No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATGACATATCCCATGAA4680.13679290781385642No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG4640.13562373766160124No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC4590.13416227497128227No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4560.13328539735709088No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATA4520.13211622720483568No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4430.12948559436226154No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4420.12919330182419772No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA4400.12860871674807015No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT4380.12802413167194254No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAGGTTAATTATGCCTGCTAGGTT4270.1248089137532408No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4230.1236397436009856No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4080.11925535553002867No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA4070.11896306299196487No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4050.11837847791583728No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACATTGCTGAGAGTCCAA4050.11837847791583728No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4040.1180861853777735No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATATT4030.1177938928397097No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4020.11750160030164589No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG3990.11662472268745452No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT3990.11662472268745452No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC3970.11604013761132692No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG3970.11604013761132692No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT3900.11399408984488035No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG3860.11282491969262516No Hit
TAATTAACCTTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATAT3840.11224033461649757No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT3810.11136345700230618No Hit
GGATAAAACCTAGCAGGCATAATTAACCTTAATCTCCTTTTTTCATTAAC3800.1110711644642424No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC3770.110194286850051No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3750.10960970177392342No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG3680.10756365400747683No Hit
GCTTTAGGGCATGGACATCGACCCGTATAAAGAATTTGGAGCTTCTGTGG3680.10756365400747683No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3660.10697906893134924No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3610.10551760624103028No Hit
CCCTAGCAGCCATGGGAAGGATGTGTATTTCCGAGAGAGGACAACTGAGT3610.10551760624103028No Hit
GGCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3560.1040561435507113No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3550.1037638510126475No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC3520.10288697339845611No Hit
CCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGC3520.10288697339845611No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3500.10230238832232852No Hit
GTATACATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAAC3500.10230238832232852No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3490.10201009578426473No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3490.10201009578426473No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG3480.10171780324620093No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC3460.10113321817007334No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3460.10113321817007334No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3460.10113321817007334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)