FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429701
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45811.0660901417497284No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15700.3653703389100793No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10020.23318540101140095No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9690.2255056422954566No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACCGTTAATGAAAAA9350.21759316361842307No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8970.2087498050970326No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTTAGGAAAGGAGGGAGTTTG8940.2080516452137649No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8670.2017682062643559No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8390.19525204735385768No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTAAC8260.1922266878596978No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7510.1747726907780061No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7470.17384181093364923No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7430.17291093108929234No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7260.16895469175077554No Hit
CTTATATAGAACGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7200.1675583719842402No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6940.1615076529959204No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6880.1601113332293851No Hit
CGATAATATGGGCCTAAAAACCAGACAACTATTGTGGTTTCACATTTCTT6870.15987861326829586No Hit
ATTCTATGTTGGGTTGAGTTAATGAATCTGGCCACCTGGGTGGGAGGTAA6660.15499149408542218No Hit
CTCTTACCGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6280.14614813556403172No Hit
ATATTATCGTTGACATATCTGACTACTAATTCCCTGGATGCTTGGTCTTC6200.14428637587531795No Hit
AGTCTGTACAACATCTTGAGGCCCTTTTTACCTCTATTACCAATTTTCTT6110.14219189622551495No Hit
CTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACAT6060.14102829642006884No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5750.13381397762630295No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5690.1324176578597676No Hit
CTCTTATATAGAACGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5650.1314867780154107No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGTTACTCCCTTAACTTCAT5600.1303231782099646No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5440.12659965883253704No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC5070.1179890202722358No Hit
CTTTTACACAATGCGGCTATCCGGCCTTGATGCCTTTATATGCATGTATA5070.1179890202722358No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5020.1168254204667897No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTC4990.11612726058352205No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4970.11566182066134359No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAGCGTTGGGGTTACT4920.11449822085589746No Hit
GGGAATTAGTAGTCAGATATGTCAACGATAATATGGGCCTAAAAACCAGA4890.1138000609726298No Hit
CTTTTTACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAA4850.11286918112827292No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4840.1126364611671837No Hit
CTTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4770.11100742143955913No Hit
TAATAAAACCAAGCGTTGGGGTTACTCCCTTAACTTCATGGGATATGTAA4750.1105419815173807No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAAT4730.11007654159520225No Hit
GTACAACATCTTGAGGCCCTTTTTACCTCTATTACCAATTTTCTTCTGTC4730.11007654159520225No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4610.10728390206213158No Hit
GTACTAAAGATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4580.10658574217886391No Hit
GTATACATGCATATAAAGGCATCAAGGCCGGATAGCCGCATTGTGTAAAA4570.10635302221777469No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA4570.10635302221777469No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT4410.10262950284034712No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA4390.1021640629181687No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4340.10100046311272257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)