FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429701
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45631.0619011824501223No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16960.3946930540073214No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACCGTTAATGAAAAA9850.22922916167288418No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9650.22457476245109972No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTTAGGAAAGGAGGGAGTTTG9340.2173604436573338No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8950.20828436517485416No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8870.2064226054861404No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8480.1973465270036607No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTAAC7960.1852450890270211No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7850.18268516945503965No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7730.179892529921969No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7660.17826349019434443No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7520.1750054107390953No Hit
CTCTTACCGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7470.17384181093364923No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7330.17058373147840009No Hit
CTTATATAGAACGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7020.1633694126846342No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6840.1591804533850282No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6760.1573186936963144No Hit
CTCTTATATAGAACGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6470.15056981482472698No Hit
ATTCTATGTTGGGTTGAGTTAATGAATCTGGCCACCTGGGTGGGAGGTAA6320.14707901540838864No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6290.14638085552512095No Hit
CTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACAT6230.14498453575858564No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5970.13893381677026584No Hit
CGATAATATGGGCCTAAAAACCAGACAACTATTGTGGTTTCACATTTCTT5900.1373047770426413No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5760.13404669758739216No Hit
ATATTATCGTTGACATATCTGACTACTAATTCCCTGGATGCTTGGTCTTC5720.13311581774303527No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGTTACTCCCTTAACTTCAT5720.13311581774303527No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5390.12543605902709093No Hit
CTTTTTACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAA5380.1252033390660017No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTC5330.1240397392605556No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5260.12241069953293104No Hit
GTACAACATCTTGAGGCCCTTTTTACCTCTATTACCAATTTTCTTCTGTC5220.12147981968857414No Hit
CTTTTACACAATGCGGCTATCCGGCCTTGATGCCTTTATATGCATGTATA5050.11752358035005737No Hit
AGTCTGTACAACATCTTGAGGCCCTTTTTACCTCTATTACCAATTTTCTT5020.1168254204667897No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4960.11542910070025438No Hit
ATATTGTACTAAAGATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA4940.11496366077807592No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA4930.1147309408169867No Hit
GTACTAAAGATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4920.11449822085589746No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAAT4920.11449822085589746No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC4840.1126364611671837No Hit
GTATACATGCATATAAAGGCATCAAGGCCGGATAGCCGCATTGTGTAAAA4830.11240374120609446No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAGCGTTGGGGTTACT4780.11124014140064836No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4750.1105419815173807No Hit
GGGAATTAGTAGTCAGATATGTCAACGATAATATGGGCCTAAAAACCAGA4740.11030926155629146No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4680.10891294178975612No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT4600.10705118210104236No Hit
GGAACATATTGTACTAAAGATCAAGCAATGTTTTCGGAAACTGCCTGTAA4500.10472398249015012No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGGCCCTTTTTACC4430.10309494276252557No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4390.1021640629181687No Hit
TAATAAAACCAAGCGTTGGGGTTACTCCCTTAACTTCATGGGATATGTAA4330.10076774315163335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)