FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522919
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43310.8282353481131877No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG34800.6654950384285139No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT23940.4578146902292707No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA14710.28130551768055856No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13920.26619801537140547No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG13670.26141716021028116No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12740.24363237901089846No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10370.1983098720834393No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9690.185305946045181No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9510.18186373032917144No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCT9030.17268448841981263No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9020.17249325421336764No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8700.16637375960712847No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATG8400.16063673341377918No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8310.15891562555577443No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT8110.1550909414268749No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8010.15317859936242517No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7970.15241366253664526No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7840.14992761785286057No Hit
ACATTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCC7740.14801527578841084No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7690.14705910475618594No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7550.1443818258659563No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7510.1436168890401764No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7490.14323442062728645No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7490.14323442062728645No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7460.14266071800795152No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA7170.13711492602104725No Hit
ATATTGTACTAAAGCTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA7140.1365412234017123No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6780.1296567919696932No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6570.12564087363434873No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6390.12219865791833917No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT6190.11837397378943967No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6020.1151229922798751No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6000.11474052386698515No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5990.11454928966054016No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5720.10938596608652583No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5680.10862102926074593No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5660.10823856084785598No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5600.10709115560918613No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5420.10364893989317657No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5400.10326647148028661No Hit
GGAACATATTGTACTAAAGCTCAAGCAATGTTTTCGGAAACTGCCTGTAA5380.10288400306739667No Hit
ACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAATTGTAAGA5280.10097166100294691No Hit
GTGCTGGTGCACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5250.100397958383612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)