FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433284
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC27930.6446118481180935No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21880.504980567018399No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGCTTGGAAGTAATGATTAACTAGA21040.4855937445186067No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA15470.35704064770450794No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA14190.32749882294291965No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC12340.2848016543421867No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTGTCTAAATTTTAT11980.27649301612799No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC11810.27256949252684154No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10640.24556641833070225No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTACGATGTGTT10570.2439508497890529No Hit
GTGTAAATAGTGTCTAGCTTGGAAGTAATGATTAACTAGATGTTCTGGAT10420.24048891719980428No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT10220.2358730070808061No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT9880.22802595987850924No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG9820.2266411868428098No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC9120.21048550142631622No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8500.1961761800574219No Hit
TCCTAGTACAAAGACCTGTAATCTAATCTCCTCCCCCAACTCCTCCCAGT8310.19179106544437366No Hit
GTACAAAGACCTGTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA8260.1906370879146241No Hit
AATTTAGACAGTAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATA8180.18879072386702486No Hit
CTTCTAGATACTGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA7960.1837132227361269No Hit
AGTTTGCAGCACAGCCTAGCAGCCATGGAAACGACGTATATTTGCGGGAG7910.18255924520637734No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA7660.17678935755762964No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7650.17655856205167972No Hit
GCCTAGCAGCCATGGAAACGACGTATATTTGCGGGAGAGGACAACAGAGT7620.17586617553383No Hit
ATACTGCACTCAGGCAAGCTATTCTTTGCTGGGGGGACTTAATGACTCTA7510.17332742496838102No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7370.1700962878850823No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7300.16848071934343295No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG7300.16848071934343295No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7230.16686515080178357No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7120.1643264002363346No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCAGTATCTAGAAGATCTCGTA6990.16132605865898578No Hit
GTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGT6790.15671014853998763No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6770.15624855752808783No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6530.15070946538529004No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTGTAATCTAATCTCCTCC6530.15070946538529004No Hit
GTGCAGTATGGTGCGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGA6500.1500170788674403No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6490.14978628336149039No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6470.1493246923495906No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACAGACT6460.14909389684364066No Hit
GTCTAAATTTTATGGGTTATGTCATTGGATGTCATGGGTCCTTGCCACAA6380.1472475327960414No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6350.1465551462781917No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG6320.14586275976034196No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG6300.1454011687484421No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT6250.1442471912186926No Hit
CTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAAC6130.1414776451472937No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTGTAATCTAATCT6090.14055446312349404No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6040.13940048559374452No Hit
ACTACATACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGT5940.13709253053424542No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACT5890.1359385530044959No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAAATCTTGCCC5800.13386139345094672No Hit
GGTTTGATACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGT5790.1336305979449968No Hit
CTCTATGGAAGGCGGGTATATTATATAAAAGAGAAACAACACATAGCGCC5690.13132264288549775No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT5660.13063025636764802No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT5650.1303994608616981No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5650.1303994608616981No Hit
TCTAGATACTGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC5630.1299378698497983No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTAT5580.12878389232004875No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5570.12855309681409885No Hit
AATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTTGTCT5560.12832230130814892No Hit
CATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT5560.12832230130814892No Hit
TAACCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG5540.12786071029624912No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGT5520.1273991192843493No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGGGC5480.12647593726054968No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCGCACCATACTG5460.12601434624864985No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG5390.12439877770700049No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG5380.12416798220105058No Hit
GTATGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG5380.12416798220105058No Hit
TCCCGATACAGAGCTGAGGCAGTATCTAGAAGATCTCGTACTGAAGGAAA5340.12324480017725095No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCGCACCATACTGCACTCAGGCA5320.12278320916535113No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC5280.1218600271415515No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAGCT5260.12139843612965168No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5250.12116764062370178No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5150.11885968556420269No Hit
CTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTACGTCCCGTCGGCG5150.11885968556420269No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5140.11862889005825278No Hit
CATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT5110.11793650354040305No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5060.11678252601065352No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGAATTGTGGGTCT5000.11539775297495407No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4960.11447457095115443No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4950.11424377544520453No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA4870.11239741139760526No Hit
GTATGCATGTATTCAATCGAAGCAGGCTTTCACTTTCTCGCCAACTTACA4830.11147422937380563No Hit
GACCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4830.11147422937380563No Hit
ATATTTACCATTGGACAAGGGTATTAAACCTTATTATCCAGAACATCTAG4820.11124343386785572No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGAATTGTGGGTCTTTTGGGTTTT4820.11124343386785572No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4800.11078184285595591No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA4790.110551047350006No Hit
ACACTAATGTGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCT4750.10962786532620636No Hit
GACCCATGACATCCAATGACATAACCCATAAAATTTAGACAGTAACCCCA4730.10916627431430655No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4730.10916627431430655No Hit
ACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATT4680.10801229678455701No Hit
CTTTCACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4650.10731991026670729No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGCTTGGA4600.10616593273695774No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4550.1050119552072082No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGCTCCACGCATGCGCTGATGGCC4530.10455036419530839No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4520.10431956868935847No Hit
GACCTGTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4500.10385797767745866No Hit
CCTGTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG4480.10339638666555884No Hit
ATCCAAATGTTACCAAATATTTACCATTGGACAAGGGTATTAAACCTTAT4480.10339638666555884No Hit
CCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTT4440.10247320464175921No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4400.10155002261795958No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4390.10131922711200966No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG4370.10085763610010985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)