FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521429
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15640.2999449589493488No Hit
CTTATATAGAATCCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15260.2926572937063339No Hit
CATTTAAACCCTAACAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT14880.2853696284633191No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14830.2844107251418697No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT14590.279807989198913No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAA14250.2732874466130576No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG12060.23128748113357714No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11360.21786283463328662No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10780.20673955610447442No Hit
GTGTAAAAGGGGCAACAAAGCCCAAAAGACCCACAATTCTTTGACATACT10760.20635599477589472No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10210.19580805823995215No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10130.1942738129256332No Hit
CTCTTATATAGAATCCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10110.1938902515970535No Hit
GAAAAGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGTATA9940.19062998030412578No Hit
CTTTTACACAATGTGGATATCCTGCCTTGATGCCTTTATATGCATGTATA9790.18775327033977782No Hit
ATCTCAATGTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGG9480.18180806974679198No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9430.18084916642534268No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9350.17931492111102376No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9150.17547930782522644No Hit
TATCCACATTGTGTAAAAGGGGCAACAAAGCCCAAAAGACCCACAATTCT9050.17356150118232777No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG8860.16991766856082036No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT8720.16723273926076226No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT8560.16416424863212442No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8120.15572589940337037No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8020.1538080927604717No Hit
GTGTCAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC8020.1538080927604717No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC8020.1538080927604717No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCA7980.15304097010331225No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7960.15265740877473252No Hit
CATATAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAAGGGGCAACA7900.15150672478899332No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA7840.15035604080325413No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC7570.1451779628674278No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7280.1396163236030217No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT7060.1353971489886447No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA6610.12676701909560073No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT6590.126383457767021No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA6520.12504099311699196No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6340.1215889411597744No Hit
GGATATCCACATTGTGTAAAAGGGGCAACAAAGCCCAAAAGACCCACAAT6270.12024647650974533No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT6150.11794510853826695No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA6120.11736976654539737No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTGTTAGGGTTTAAATGTATA6040.11583552123107844No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6020.11545195990249871No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATATCCACAT5940.11391771458817979No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATATCCACATTGTGT5940.11391771458817979No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTCAGGCA5900.11315059193102034No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC5820.11161634661670142No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5660.10854785598806357No Hit
ACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5650.10835607532377371No Hit
ATTTACATACTCTGTGGAAGGCTGGGATTCTATATAAGAGAGAAACTACA5630.10797251399519397No Hit
GTGGATATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG5620.1077807333309041No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5600.10739717200232439No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5540.10624648801658518No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5530.10605470735229533No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5520.10586292668800547No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5490.10528758469513587No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5470.10490402336655613No Hit
ATGTGGATATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGC5440.10432868137368655No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5420.10394512004510681No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5280.1012601907450487No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5270.10106841008075884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)